Simultaneous patterning of proteins and cells through bioconjugation with photoreactable phospholipid polymers

Ji Hun Seo; Kazuhiko Ishihara

doi:10.1039/c7ra07389e

Simultaneous patterning of proteins and cells through bioconjugation with photoreactable phospholipid polymers

Ji Hun Seo, Kazuhiko Ishihara

Research output: Contribution to journal › Article › peer-review

Abstract

Photoreactable bioconjugated macromolecules were synthesized via atom transfer radical polymerization (ATRP), using an N-hydroxysuccinimide ester functional end group with the anti-biofouling 2-methacryloyloxyethyl phosphorylcholine (MPC) units and photoreactable 2-(4-azidobenzamido)ethyl methacrylate units. The polymer was then conjugated with bovine serum albumin (BSA), and the simultaneous patterning of BSA and L-929 fibroblast cells was prepared by a simple coating and irradiation (254 nm UV) process.

Original language	English
Pages (from-to)	40669-40672
Number of pages	4
Journal	RSC Advances
Volume	7
Issue number	64
DOIs	https://doi.org/10.1039/c7ra07389e
Publication status	Published - 2017

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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abstract = "Photoreactable bioconjugated macromolecules were synthesized via atom transfer radical polymerization (ATRP), using an N-hydroxysuccinimide ester functional end group with the anti-biofouling 2-methacryloyloxyethyl phosphorylcholine (MPC) units and photoreactable 2-(4-azidobenzamido)ethyl methacrylate units. The polymer was then conjugated with bovine serum albumin (BSA), and the simultaneous patterning of BSA and L-929 fibroblast cells was prepared by a simple coating and irradiation (254 nm UV) process.",

author = "Seo, {Ji Hun} and Kazuhiko Ishihara",

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AB - Photoreactable bioconjugated macromolecules were synthesized via atom transfer radical polymerization (ATRP), using an N-hydroxysuccinimide ester functional end group with the anti-biofouling 2-methacryloyloxyethyl phosphorylcholine (MPC) units and photoreactable 2-(4-azidobenzamido)ethyl methacrylate units. The polymer was then conjugated with bovine serum albumin (BSA), and the simultaneous patterning of BSA and L-929 fibroblast cells was prepared by a simple coating and irradiation (254 nm UV) process.

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U2 - 10.1039/c7ra07389e

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Simultaneous patterning of proteins and cells through bioconjugation with photoreactable phospholipid polymers

Abstract

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