Simultaneous patterning of proteins and cells through bioconjugation with photoreactable phospholipid polymers

Ji-Hun Seo, Kazuhiko Ishihara

Research output: Contribution to journalArticle

Abstract

Photoreactable bioconjugated macromolecules were synthesized via atom transfer radical polymerization (ATRP), using an N-hydroxysuccinimide ester functional end group with the anti-biofouling 2-methacryloyloxyethyl phosphorylcholine (MPC) units and photoreactable 2-(4-azidobenzamido)ethyl methacrylate units. The polymer was then conjugated with bovine serum albumin (BSA), and the simultaneous patterning of BSA and L-929 fibroblast cells was prepared by a simple coating and irradiation (254 nm UV) process.

Original languageEnglish
Pages (from-to)40669-40672
Number of pages4
JournalRSC Advances
Volume7
Issue number64
DOIs
Publication statusPublished - 2017 Jan 1

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Biofouling
Atom transfer radical polymerization
Phospholipids
Fibroblasts
Bovine Serum Albumin
Macromolecules
Esters
Polymers
Cells
Irradiation
Proteins
Coatings
Methacrylates
N-hydroxysuccinimide
2-methacryloyloxyethyl phosphorylcholine

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Simultaneous patterning of proteins and cells through bioconjugation with photoreactable phospholipid polymers. / Seo, Ji-Hun; Ishihara, Kazuhiko.

In: RSC Advances, Vol. 7, No. 64, 01.01.2017, p. 40669-40672.

Research output: Contribution to journalArticle

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