Simultaneous pretreatment and saccharification: Green technology for enhanced sugar yields from biomass using a fungal consortium

Saurabh Sudha Dhiman, Jung Rim Haw, Dayanand Kalyani, Vipin C. Kalia, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.

Original languageEnglish
Pages (from-to)50-57
Number of pages8
JournalBioresource Technology
Volume179
DOIs
Publication statusPublished - 2015 Mar 1

Fingerprint

Environmental technology
Saccharification
Sugars
straw
sugar
Biomass
rice
Salix
biomass
Straw
atomic force microscopy
hydrolysis
enzyme
Hazardous Substances
substrate
Laccase
Bioethanol
methodology
Conversion efficiency
Hydrolysis

Keywords

  • Ethanol
  • Fungal consortia
  • Pretreatment
  • Reducing sugars
  • Saccharification

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Simultaneous pretreatment and saccharification : Green technology for enhanced sugar yields from biomass using a fungal consortium. / Dhiman, Saurabh Sudha; Haw, Jung Rim; Kalyani, Dayanand; Kalia, Vipin C.; Kang, Yun Chan; Lee, Jung Kul.

In: Bioresource Technology, Vol. 179, 01.03.2015, p. 50-57.

Research output: Contribution to journalArticle

Dhiman, Saurabh Sudha ; Haw, Jung Rim ; Kalyani, Dayanand ; Kalia, Vipin C. ; Kang, Yun Chan ; Lee, Jung Kul. / Simultaneous pretreatment and saccharification : Green technology for enhanced sugar yields from biomass using a fungal consortium. In: Bioresource Technology. 2015 ; Vol. 179. pp. 50-57.
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