Simultaneous production of endoglucanase and β-glucosidase using synthetic two cistron genes

Young Do Yoo, M. Y. Pack

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Simultaneous production of endoglucanase and β-glucosidase by using a synthetic two cistron system in Escherichia coli was attempted as a possible way of reducing production cost. The first cistron in this system we constructed is an endoglucanase gene fused to a tac promoter that provides for efficient expression. The second cistron is a β-glucosidase structural gene. A ribosome binding site sequence of 33-base was inserted between the two cistron genes. E. coli cells transformed with the system produced 12.4 units/mg protein of endoglucanase and 327 units/mg protein of β-glucosidase, which represent 15% and 22% of total cellular protein, respectively, in L medium within three hours after induction with IPTG.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalBiotechnology Letters
Volume14
Issue number2
DOIs
Publication statusPublished - 1992 Feb 1
Externally publishedYes

Fingerprint

Glucosidases
Cellulase
Genes
Proteins
Escherichia coli
Isopropyl Thiogalactoside
Binding sites
Binding Sites
Costs
Ribosomes
Costs and Cost Analysis

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Biotechnology

Cite this

Simultaneous production of endoglucanase and β-glucosidase using synthetic two cistron genes. / Yoo, Young Do; Pack, M. Y.

In: Biotechnology Letters, Vol. 14, No. 2, 01.02.1992, p. 77-82.

Research output: Contribution to journalArticle

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