Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip

Jung Kyung Kim, Hyunwoo Bang, Seok Chung, Alexey Dan Chin-Yu, Chanil Chung, Han Sang Jo, Jung Yul Yoo, Jun Keun Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Flow cytometry and microfluidic system are versatile tools to study the functional genomics at the single-cell level in the postgenomic era. We have developed a microfluidics-based benchtop flow cytometry system incorporating a disposable plastic microchip and low-power diode lasers. The plastic microfluidic chip is designed and fabricated using soft lithography which enables the development of inexpensive and flexible miniaturized fluidic devices. The microchip contains the hydrodyamic focusing chamber where the sample and sheath fluids are driven by a pressure difference. The performance of the combined fluidics and optics is studied systematically to evaluate the detection accuracy and efficiency of our flow cytometry system. The interactions of the biological particles with surrounding squeezed flow and focused laser beam are investigated to optimize the design of the microchannel network as well as the optical characteristics of the instrument for efficient single-cell manipulation and detection.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsH. Becker, P. Woias
Pages8-20
Number of pages13
Volume4982
DOIs
Publication statusPublished - 2003
Externally publishedYes
EventMicrofluidics, BioMEMS, and Medical Microsystems - San Jose, CA, United States
Duration: 2003 Jan 272003 Jan 29

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems
CountryUnited States
CitySan Jose, CA
Period03/1/2703/1/29

Fingerprint

cytometry
Flow cytometry
Microfluidics
manipulators
plastics
Fluorescence
chips
Plastics
fluorescence
fluidics
cells
Fluidic devices
Fluidics
Microchannels
Lithography
Laser beams
Semiconductor lasers
Optics
microchannels
sheaths

Keywords

  • Bio-MEMS
  • Flow cytometry
  • Hydrodynamic focusing
  • Lab-on-a-chip
  • Microchip
  • Microfabrication
  • Microfluidics
  • Microsystem
  • Single-cell manipulation
  • Soft lithography

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kim, J. K., Bang, H., Chung, S., Chin-Yu, A. D., Chung, C., Jo, H. S., ... Chang, J. K. (2003). Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip. In H. Becker, & P. Woias (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4982, pp. 8-20) https://doi.org/10.1117/12.472741

Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip. / Kim, Jung Kyung; Bang, Hyunwoo; Chung, Seok; Chin-Yu, Alexey Dan; Chung, Chanil; Jo, Han Sang; Yoo, Jung Yul; Chang, Jun Keun.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / H. Becker; P. Woias. Vol. 4982 2003. p. 8-20.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kim, JK, Bang, H, Chung, S, Chin-Yu, AD, Chung, C, Jo, HS, Yoo, JY & Chang, JK 2003, Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip. in H Becker & P Woias (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4982, pp. 8-20, Microfluidics, BioMEMS, and Medical Microsystems, San Jose, CA, United States, 03/1/27. https://doi.org/10.1117/12.472741
Kim JK, Bang H, Chung S, Chin-Yu AD, Chung C, Jo HS et al. Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip. In Becker H, Woias P, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4982. 2003. p. 8-20 https://doi.org/10.1117/12.472741
Kim, Jung Kyung ; Bang, Hyunwoo ; Chung, Seok ; Chin-Yu, Alexey Dan ; Chung, Chanil ; Jo, Han Sang ; Yoo, Jung Yul ; Chang, Jun Keun. / Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip. Proceedings of SPIE - The International Society for Optical Engineering. editor / H. Becker ; P. Woias. Vol. 4982 2003. pp. 8-20
@inproceedings{fa4c9e2017ca4101a551e76aca789736,
title = "Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip",
abstract = "Flow cytometry and microfluidic system are versatile tools to study the functional genomics at the single-cell level in the postgenomic era. We have developed a microfluidics-based benchtop flow cytometry system incorporating a disposable plastic microchip and low-power diode lasers. The plastic microfluidic chip is designed and fabricated using soft lithography which enables the development of inexpensive and flexible miniaturized fluidic devices. The microchip contains the hydrodyamic focusing chamber where the sample and sheath fluids are driven by a pressure difference. The performance of the combined fluidics and optics is studied systematically to evaluate the detection accuracy and efficiency of our flow cytometry system. The interactions of the biological particles with surrounding squeezed flow and focused laser beam are investigated to optimize the design of the microchannel network as well as the optical characteristics of the instrument for efficient single-cell manipulation and detection.",
keywords = "Bio-MEMS, Flow cytometry, Hydrodynamic focusing, Lab-on-a-chip, Microchip, Microfabrication, Microfluidics, Microsystem, Single-cell manipulation, Soft lithography",
author = "Kim, {Jung Kyung} and Hyunwoo Bang and Seok Chung and Chin-Yu, {Alexey Dan} and Chanil Chung and Jo, {Han Sang} and Yoo, {Jung Yul} and Chang, {Jun Keun}",
year = "2003",
doi = "10.1117/12.472741",
language = "English",
volume = "4982",
pages = "8--20",
editor = "H. Becker and P. Woias",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip

AU - Kim, Jung Kyung

AU - Bang, Hyunwoo

AU - Chung, Seok

AU - Chin-Yu, Alexey Dan

AU - Chung, Chanil

AU - Jo, Han Sang

AU - Yoo, Jung Yul

AU - Chang, Jun Keun

PY - 2003

Y1 - 2003

N2 - Flow cytometry and microfluidic system are versatile tools to study the functional genomics at the single-cell level in the postgenomic era. We have developed a microfluidics-based benchtop flow cytometry system incorporating a disposable plastic microchip and low-power diode lasers. The plastic microfluidic chip is designed and fabricated using soft lithography which enables the development of inexpensive and flexible miniaturized fluidic devices. The microchip contains the hydrodyamic focusing chamber where the sample and sheath fluids are driven by a pressure difference. The performance of the combined fluidics and optics is studied systematically to evaluate the detection accuracy and efficiency of our flow cytometry system. The interactions of the biological particles with surrounding squeezed flow and focused laser beam are investigated to optimize the design of the microchannel network as well as the optical characteristics of the instrument for efficient single-cell manipulation and detection.

AB - Flow cytometry and microfluidic system are versatile tools to study the functional genomics at the single-cell level in the postgenomic era. We have developed a microfluidics-based benchtop flow cytometry system incorporating a disposable plastic microchip and low-power diode lasers. The plastic microfluidic chip is designed and fabricated using soft lithography which enables the development of inexpensive and flexible miniaturized fluidic devices. The microchip contains the hydrodyamic focusing chamber where the sample and sheath fluids are driven by a pressure difference. The performance of the combined fluidics and optics is studied systematically to evaluate the detection accuracy and efficiency of our flow cytometry system. The interactions of the biological particles with surrounding squeezed flow and focused laser beam are investigated to optimize the design of the microchannel network as well as the optical characteristics of the instrument for efficient single-cell manipulation and detection.

KW - Bio-MEMS

KW - Flow cytometry

KW - Hydrodynamic focusing

KW - Lab-on-a-chip

KW - Microchip

KW - Microfabrication

KW - Microfluidics

KW - Microsystem

KW - Single-cell manipulation

KW - Soft lithography

UR - http://www.scopus.com/inward/record.url?scp=0037721716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037721716&partnerID=8YFLogxK

U2 - 10.1117/12.472741

DO - 10.1117/12.472741

M3 - Conference contribution

VL - 4982

SP - 8

EP - 20

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Becker, H.

A2 - Woias, P.

ER -