Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip

Jung Kyung Kim, Hyunwoo Bang, Seok Chung, Alexey Dan Chin-Yu, Chanil Chung, Han Sang Jo, Jung Yul Yoo, Jun Keun Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Citations (Scopus)

Abstract

Flow cytometry and microfluidic system are versatile tools to study the functional genomics at the single-cell level in the postgenomic era. We have developed a microfluidics-based benchtop flow cytometry system incorporating a disposable plastic microchip and low-power diode lasers. The plastic microfluidic chip is designed and fabricated using soft lithography which enables the development of inexpensive and flexible miniaturized fluidic devices. The microchip contains the hydrodyamic focusing chamber where the sample and sheath fluids are driven by a pressure difference. The performance of the combined fluidics and optics is studied systematically to evaluate the detection accuracy and efficiency of our flow cytometry system. The interactions of the biological particles with surrounding squeezed flow and focused laser beam are investigated to optimize the design of the microchannel network as well as the optical characteristics of the instrument for efficient single-cell manipulation and detection.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsH. Becker, P. Woias
Pages8-20
Number of pages13
Volume4982
DOIs
Publication statusPublished - 2003
Externally publishedYes
EventMicrofluidics, BioMEMS, and Medical Microsystems - San Jose, CA, United States
Duration: 2003 Jan 272003 Jan 29

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems
CountryUnited States
CitySan Jose, CA
Period03/1/2703/1/29

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Keywords

  • Bio-MEMS
  • Flow cytometry
  • Hydrodynamic focusing
  • Lab-on-a-chip
  • Microchip
  • Microfabrication
  • Microfluidics
  • Microsystem
  • Single-cell manipulation
  • Soft lithography

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kim, J. K., Bang, H., Chung, S., Chin-Yu, A. D., Chung, C., Jo, H. S., Yoo, J. Y., & Chang, J. K. (2003). Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip. In H. Becker, & P. Woias (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4982, pp. 8-20) https://doi.org/10.1117/12.472741