Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles

Sung Gil Hong, Byoung Chan Kim, Hyon Bin Na, Jinwoo Lee, Jongkyu Youn, Seung Wook Chung, Chang Won Lee, Byoungsoo Lee, Han Sol Kim, Erik Hsiao, Seong H. Kim, Byung Gee Kim, Hyun Gyu Park, Ho Nam Chang, Taeghwan Hyeon, Jonathan S. Dordick, Jay W. Grate, Jungbae Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

For the encapsulation of biomolecules in inorganic materials, we have developed a unique enzyme-silicate conjugate material that consists of a self-assembled molecularly thin silicate layer on the surface of each individual enzyme molecule. The enzyme-silicate conjugate materials, called single enzyme caged nanoparticles (SECNs), were synthesized via the silica polymerization on the surface of enzyme molecule after solubilizing each enzyme molecule in hexane by using a tiny amount of surfactant, called “ion-pairing”. SECNs possess near native enzyme activity in aqueous media with minimal substrate diffusional limitations, and are highly stable under the protection of silicate network cage. Due to their nearly molecular size, SECNs can also be adsorbed into mesoporous silica materials to yield robust and easily-recyclable enzymatic systems that can be used in a number of potential biocatalytic applications such as diagnostics, biosensors, biotransformations, biofuel production, bioremediation and CO2 capture.

Original languageEnglish
Pages (from-to)510-515
Number of pages6
JournalChemical Engineering Journal
Volume322
DOIs
Publication statusPublished - 2017

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Keywords

  • Enzyme catalysis
  • Enzyme immobilization
  • Enzyme stabilization
  • Enzymes
  • Single enzyme caged nanoparticles

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Hong, S. G., Kim, B. C., Na, H. B., Lee, J., Youn, J., Chung, S. W., Lee, C. W., Lee, B., Kim, H. S., Hsiao, E., Kim, S. H., Kim, B. G., Park, H. G., Chang, H. N., Hyeon, T., Dordick, J. S., Grate, J. W., & Kim, J. (2017). Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles. Chemical Engineering Journal, 322, 510-515. https://doi.org/10.1016/j.cej.2017.04.022