Single enzyme nanoparticles in nanoporous silica: A hierarchical approach to enzyme stabilization and immobilization

Jungbae Kim; Hongfei Jia; Chang won Lee; Seung wook Chung; Ja Hun Kwak; Yongsoon Shin; Alice Dohnalkova; Byung Gee Kim; Ping Wang; Jay W. Grate

doi:10.1016/j.enzmictec.2005.11.042

Single enzyme nanoparticles in nanoporous silica: A hierarchical approach to enzyme stabilization and immobilization

Jungbae Kim, Hongfei Jia, Chang won Lee, Seung wook Chung, Ja Hun Kwak, Yongsoon Shin, Alice Dohnalkova, Byung Gee Kim, Ping Wang, Jay W. Grate

Research output: Contribution to journal › Article

50 Citations (Scopus)

Abstract

Single enzyme nanoparticles of α-chymotrypsin (SEN-CT), in which each CT molecule is surrounded by a thin polymeric organic/inorganic network, stabilized the CT activity in a shaking condition as well as in a non-shaking condition. Since SEN-CT is soluble in a buffer solution and less than 10 nm in size, SEN-CT could be further immobilized in nanoporous silica with an average pore size of 29 nm. Free CT and SEN-CT were immobilized in nanoporous silica (NPS), and nanoporous silica that was first silanized with aminopropyltriethoxysilane (amino-NPS) to generate a positive surface charge. The SEN-CT adsorbed in amino-NPS was more stable than CT immobilized by either adsorption in NPS or covalent bonding to amino-NPS. In shaking conditions, nanoporous silica provided an additional stabilization by protecting SEN-CT from shear stresses. At 22 °C with harsh shaking, free, NPS-adsorbed and NPS-covalently attached CT showed half lives of 1, 62, and 80 h, respectively; whereas SEN-CT adsorbed in amino-NPS showed no activity loss within 12 days. The combination of SENs and nanoporous silica, which makes an active and stable immobilized enzyme system, represents a new structure for biocatalytic applications.

Original language	English
Pages (from-to)	474-480
Number of pages	7
Journal	Enzyme and Microbial Technology
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/j.enzmictec.2005.11.042
Publication status	Published - 2006 Jul 3
Externally published	Yes

Keywords

Enzyme immobilization
Enzyme stabilization
Mesocellular foams
Nanoporous silica
Single enzyme nanoparticles
α-Chymotrypsin

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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Kim, J., Jia, H., Lee, C. W., Chung, S. W., Kwak, J. H., Shin, Y., Dohnalkova, A., Kim, B. G., Wang, P., & Grate, J. W. (2006). Single enzyme nanoparticles in nanoporous silica: A hierarchical approach to enzyme stabilization and immobilization. Enzyme and Microbial Technology, 39(3), 474-480. https://doi.org/10.1016/j.enzmictec.2005.11.042

Single enzyme nanoparticles in nanoporous silica : A hierarchical approach to enzyme stabilization and immobilization. / Kim, Jungbae; Jia, Hongfei; Lee, Chang won; Chung, Seung wook; Kwak, Ja Hun; Shin, Yongsoon; Dohnalkova, Alice; Kim, Byung Gee; Wang, Ping; Grate, Jay W.

In: Enzyme and Microbial Technology, Vol. 39, No. 3, 03.07.2006, p. 474-480.

Research output: Contribution to journal › Article

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abstract = "Single enzyme nanoparticles of α-chymotrypsin (SEN-CT), in which each CT molecule is surrounded by a thin polymeric organic/inorganic network, stabilized the CT activity in a shaking condition as well as in a non-shaking condition. Since SEN-CT is soluble in a buffer solution and less than 10 nm in size, SEN-CT could be further immobilized in nanoporous silica with an average pore size of 29 nm. Free CT and SEN-CT were immobilized in nanoporous silica (NPS), and nanoporous silica that was first silanized with aminopropyltriethoxysilane (amino-NPS) to generate a positive surface charge. The SEN-CT adsorbed in amino-NPS was more stable than CT immobilized by either adsorption in NPS or covalent bonding to amino-NPS. In shaking conditions, nanoporous silica provided an additional stabilization by protecting SEN-CT from shear stresses. At 22 °C with harsh shaking, free, NPS-adsorbed and NPS-covalently attached CT showed half lives of 1, 62, and 80 h, respectively; whereas SEN-CT adsorbed in amino-NPS showed no activity loss within 12 days. The combination of SENs and nanoporous silica, which makes an active and stable immobilized enzyme system, represents a new structure for biocatalytic applications.",

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AU - Shin, Yongsoon

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