Abstract
Single enzyme nanoparticles of α-chymotrypsin (SEN-CT), in which each CT molecule is surrounded by a thin polymeric organic/inorganic network, stabilized the CT activity in a shaking condition as well as in a non-shaking condition. Since SEN-CT is soluble in a buffer solution and less than 10 nm in size, SEN-CT could be further immobilized in nanoporous silica with an average pore size of 29 nm. Free CT and SEN-CT were immobilized in nanoporous silica (NPS), and nanoporous silica that was first silanized with aminopropyltriethoxysilane (amino-NPS) to generate a positive surface charge. The SEN-CT adsorbed in amino-NPS was more stable than CT immobilized by either adsorption in NPS or covalent bonding to amino-NPS. In shaking conditions, nanoporous silica provided an additional stabilization by protecting SEN-CT from shear stresses. At 22 °C with harsh shaking, free, NPS-adsorbed and NPS-covalently attached CT showed half lives of 1, 62, and 80 h, respectively; whereas SEN-CT adsorbed in amino-NPS showed no activity loss within 12 days. The combination of SENs and nanoporous silica, which makes an active and stable immobilized enzyme system, represents a new structure for biocatalytic applications.
| Original language | English |
|---|---|
| Pages (from-to) | 474-480 |
| Number of pages | 7 |
| Journal | Enzyme and Microbial Technology |
| Volume | 39 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 Jul 3 |
| Externally published | Yes |
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Keywords
- α-Chymotrypsin
- Enzyme immobilization
- Enzyme stabilization
- Mesocellular foams
- Nanoporous silica
- Single enzyme nanoparticles
ASJC Scopus subject areas
- Biochemistry
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Single enzyme nanoparticles in nanoporous silica : A hierarchical approach to enzyme stabilization and immobilization. / Kim, Jungbae; Jia, Hongfei; Lee, Chang won; Chung, Seung wook; Kwak, Ja Hun; Shin, Yongsoon; Dohnalkova, Alice; Kim, Byung Gee; Wang, Ping; Grate, Jay W.
In: Enzyme and Microbial Technology, Vol. 39, No. 3, 03.07.2006, p. 474-480.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single enzyme nanoparticles in nanoporous silica
T2 - A hierarchical approach to enzyme stabilization and immobilization
AU - Kim, Jungbae
AU - Jia, Hongfei
AU - Lee, Chang won
AU - Chung, Seung wook
AU - Kwak, Ja Hun
AU - Shin, Yongsoon
AU - Dohnalkova, Alice
AU - Kim, Byung Gee
AU - Wang, Ping
AU - Grate, Jay W.
PY - 2006/7/3
Y1 - 2006/7/3
N2 - Single enzyme nanoparticles of α-chymotrypsin (SEN-CT), in which each CT molecule is surrounded by a thin polymeric organic/inorganic network, stabilized the CT activity in a shaking condition as well as in a non-shaking condition. Since SEN-CT is soluble in a buffer solution and less than 10 nm in size, SEN-CT could be further immobilized in nanoporous silica with an average pore size of 29 nm. Free CT and SEN-CT were immobilized in nanoporous silica (NPS), and nanoporous silica that was first silanized with aminopropyltriethoxysilane (amino-NPS) to generate a positive surface charge. The SEN-CT adsorbed in amino-NPS was more stable than CT immobilized by either adsorption in NPS or covalent bonding to amino-NPS. In shaking conditions, nanoporous silica provided an additional stabilization by protecting SEN-CT from shear stresses. At 22 °C with harsh shaking, free, NPS-adsorbed and NPS-covalently attached CT showed half lives of 1, 62, and 80 h, respectively; whereas SEN-CT adsorbed in amino-NPS showed no activity loss within 12 days. The combination of SENs and nanoporous silica, which makes an active and stable immobilized enzyme system, represents a new structure for biocatalytic applications.
AB - Single enzyme nanoparticles of α-chymotrypsin (SEN-CT), in which each CT molecule is surrounded by a thin polymeric organic/inorganic network, stabilized the CT activity in a shaking condition as well as in a non-shaking condition. Since SEN-CT is soluble in a buffer solution and less than 10 nm in size, SEN-CT could be further immobilized in nanoporous silica with an average pore size of 29 nm. Free CT and SEN-CT were immobilized in nanoporous silica (NPS), and nanoporous silica that was first silanized with aminopropyltriethoxysilane (amino-NPS) to generate a positive surface charge. The SEN-CT adsorbed in amino-NPS was more stable than CT immobilized by either adsorption in NPS or covalent bonding to amino-NPS. In shaking conditions, nanoporous silica provided an additional stabilization by protecting SEN-CT from shear stresses. At 22 °C with harsh shaking, free, NPS-adsorbed and NPS-covalently attached CT showed half lives of 1, 62, and 80 h, respectively; whereas SEN-CT adsorbed in amino-NPS showed no activity loss within 12 days. The combination of SENs and nanoporous silica, which makes an active and stable immobilized enzyme system, represents a new structure for biocatalytic applications.
KW - α-Chymotrypsin
KW - Enzyme immobilization
KW - Enzyme stabilization
KW - Mesocellular foams
KW - Nanoporous silica
KW - Single enzyme nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=33646796717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646796717&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2005.11.042
DO - 10.1016/j.enzmictec.2005.11.042
M3 - Article
AN - SCOPUS:33646796717
VL - 39
SP - 474
EP - 480
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 3
ER -