Single gold-bridged nanoprobes for identification of single point DNA mutations

Xingyi Ma, Sojin Song, Soohyun Kim, Mi sun Kwon, Hyunsook Lee, Wounjhang Park, Sang Jun Sim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Consensus ranking of protein affinity to identify point mutations has not been established. Therefore, analytical techniques that can detect subtle variations without interfering with native biomolecular interactions are required. Here we report a rapid method to identify point mutations by a single nanoparticle sensing system. DNA-directed gold crystallization forms rod-like nanoparticles with bridges based on structural design. The nanoparticles enhance Rayleigh light scattering, achieving high refractive-index sensitivity, and enable the system to monitor even a small number of protein-DNA binding events without interference. Analysis of the binding affinity can compile an atlas to distinguish the potential of various point mutations recognized by MutS protein. We use the atlas to analyze the presence and type of single point mutations in BRCA1 from samples of human breast and ovarian cancer cell lines. The strategy of synthesis-by-design of plasmonic nanoparticles for sensors enables direct identification of subtle biomolecular binding distortions and genetic alterations.

Original languageEnglish
Article number836
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 2019 Dec 1

Fingerprint

Nanoprobes
mutations
Point Mutation
Gold
Nanoparticles
deoxyribonucleic acid
gold
nanoparticles
Atlases
DNA
proteins
affinity
Refractometry
Rayleigh scattering
structural design
ranking
DNA-Binding Proteins
Crystallization
Structural design
cultured cells

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Single gold-bridged nanoprobes for identification of single point DNA mutations. / Ma, Xingyi; Song, Sojin; Kim, Soohyun; Kwon, Mi sun; Lee, Hyunsook; Park, Wounjhang; Sim, Sang Jun.

In: Nature Communications, Vol. 10, No. 1, 836, 01.12.2019.

Research output: Contribution to journalArticle

Ma, Xingyi ; Song, Sojin ; Kim, Soohyun ; Kwon, Mi sun ; Lee, Hyunsook ; Park, Wounjhang ; Sim, Sang Jun. / Single gold-bridged nanoprobes for identification of single point DNA mutations. In: Nature Communications. 2019 ; Vol. 10, No. 1.
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