Single-shot digital holographic microscopy for quantifying a spatially-resolved Jones matrix of biological specimens

Taeseok Daniel Yang, Kwanjun Park, Yong Guk Kang, Kyoung Jin Lee, Beop-Min Kim, Youngwoon Choi

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16 Citations (Scopus)


Field-based polarization measurements are essential for the completeness of information when exploiting the complex nature of optical responses of target objects. Here, we demonstrate digital holographic microscopy for quantifying a polarization-sensitive map of an object with a single-shot measurement. Using the image-splitting device generating four different copies of an object image and a separate reference beam of an off-axis configuration enables single-shot and multi-imaging capability. With the use of two polarization filters, four complex field images containing an object's polarization response are obtained simultaneously. With this method, we can construct a complete set of 2-by-2 Jones matrix at every single point of the object's images, and thus clearly visualize the anisotropic structures of biological tissues with low level of birefringence. This method will facilitate the high-precision measurements for fast dynamics of the polarization properties of biological specimens.

Original languageEnglish
Pages (from-to)29302-29311
Number of pages10
JournalOptics Express
Issue number25
Publication statusPublished - 2016 Dec 12


ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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