We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. We have measured the path-length sensitivity of our instrument to be approximately 21 picometers Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.
|Number of pages||9|
|Publication status||Published - 2011 Apr 11|
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics