Single step isolation and activation of primary CD3 + T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers

Kwanghee Kim, Hyo Jin An, Seung Hyun Jun, Tae Jin Kim, Seon Ah Lim, Gayoung Park, Hyon Bin Na, Yong Il Park, Taeghwan Hyeon, Cassian Yee, Jeffrey A. Bluestone, Jungbae Kim, Kyung-Mi Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3 + T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8 +CD3 + T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.

Original languageEnglish
Pages (from-to)4018-4024
Number of pages7
JournalNano Letters
Volume12
Issue number8
DOIs
Publication statusPublished - 2012 Aug 8

Fingerprint

T-cells
lymphocytes
Nanofibers
isolation
alcohols
Alcohols
Chemical activation
activation
antibodies
set theory
Lymphocytes
T-Cell Antigen Receptor
Scaffolds
Polymers
Monoclonal antibodies
expansion
Monoclonal Antibodies
Nanoparticles
Ligands
Antibodies

Keywords

  • anti-CD3 monoclonal antibody
  • CD4 T lymphocytes
  • CD8 T lymphocytes
  • Electrospun magnetic nanofiber
  • immune cell therapy

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Single step isolation and activation of primary CD3 + T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers. / Kim, Kwanghee; An, Hyo Jin; Jun, Seung Hyun; Kim, Tae Jin; Lim, Seon Ah; Park, Gayoung; Na, Hyon Bin; Park, Yong Il; Hyeon, Taeghwan; Yee, Cassian; Bluestone, Jeffrey A.; Kim, Jungbae; Lee, Kyung-Mi.

In: Nano Letters, Vol. 12, No. 8, 08.08.2012, p. 4018-4024.

Research output: Contribution to journalArticle

Kim, K, An, HJ, Jun, SH, Kim, TJ, Lim, SA, Park, G, Na, HB, Park, YI, Hyeon, T, Yee, C, Bluestone, JA, Kim, J & Lee, K-M 2012, 'Single step isolation and activation of primary CD3 + T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers', Nano Letters, vol. 12, no. 8, pp. 4018-4024. https://doi.org/10.1021/nl301388d
Kim, Kwanghee ; An, Hyo Jin ; Jun, Seung Hyun ; Kim, Tae Jin ; Lim, Seon Ah ; Park, Gayoung ; Na, Hyon Bin ; Park, Yong Il ; Hyeon, Taeghwan ; Yee, Cassian ; Bluestone, Jeffrey A. ; Kim, Jungbae ; Lee, Kyung-Mi. / Single step isolation and activation of primary CD3 + T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers. In: Nano Letters. 2012 ; Vol. 12, No. 8. pp. 4018-4024.
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