Single step isolation and activation of primary CD3 + T lymphocytes using alcohol-dispersed electrospun magnetic nanofibers

Kwanghee Kim, Hyo Jin An, Seung Hyun Jun, Tae Jin Kim, Seon Ah Lim, Gayoung Park, Hyon Bin Na, Yong Il Park, Taeghwan Hyeon, Cassian Yee, Jeffrey A. Bluestone, Jungbae Kim, Kyung Mi Lee

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3 + T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8 +CD3 + T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.

Original languageEnglish
Pages (from-to)4018-4024
Number of pages7
JournalNano Letters
Volume12
Issue number8
DOIs
Publication statusPublished - 2012 Aug 8

Keywords

  • CD4 T lymphocytes
  • CD8 T lymphocytes
  • Electrospun magnetic nanofiber
  • anti-CD3 monoclonal antibody
  • immune cell therapy

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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