Single-step purified R-phycoerythrin transmits cellular imaging functionalities in vitro

Malairaj Sathuvan, Ramar Thangam, Gopal Venkateshbabu, Kit Leong Cheong, Heemin Kang, Yang Liu

Research output: Contribution to journalArticlepeer-review

Abstract

A single-step and rapid chromatographic method-based purification of Gracilaria corticata (J. Agardh) R-phycoerythrin (R-PE) was attained using polyacrylamide gel electrophoresis (PAGE) technique without affecting structural integrity. The purified R-PE had a characteristic UV–Vis spectrum with three absorbance maxima at 496, 535, and 565 nm, and fluorescence at 575 nm. R-PE was obtained with a purity index of 4.2 and a recovery yield of 44.3%. SDS-PAGE analysis exhibited three sub-units i.e., 18, 21, and 31 kDa, which corresponds to α, β, and γ, respectively. This report's purification process was considered less time-consuming and could be efficiently applied to purify phycobiliproteins. The purified R-PE showed optimal stability up to 6 h at pH 7.0 when exposed to light (3000 lx), while the temperature at which the maximum stability was retained was at 20 °C. The cellular imaging property of R-PE was effectively implemented to evaluate its credentials without affecting the cell proliferation of Vero and Hep-2 cell lines with the higher IC50 concentrations in vitro. Under fluorescence microscopy and flow cytometry analysis, purified R-PE displayed the characteristic affinity towards cell imaging functions in preliminary in vitro studies.

Original languageEnglish
Pages (from-to)563-570
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume194
DOIs
Publication statusPublished - 2022 Jan 1

Keywords

  • Gracilaria corticata
  • In vitro cell imaging
  • Phycobiliprotein
  • Physico-chemical properties
  • R-phycoerythrin
  • SDS-PAGE

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

Fingerprint

Dive into the research topics of 'Single-step purified R-phycoerythrin transmits cellular imaging functionalities in vitro'. Together they form a unique fingerprint.

Cite this