Single strand DNA breaks in T- and B-lymphocytes and granulocytes in workers exposed to benzene

Donggeun Sul, Doyoung Lee, Hosub Im, Eunha Oh, Jooja Kim, Eunil Lee

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56 Citations (Scopus)

Abstract

Comet assays were carried out to evaluate DNA damage in T- and B-lymphocytes and granulocytes from 41 workers exposed to benzene in a printing company and 41 unexposed donors. In T-lymphocytes, DNA damage was slightly higher in exposed workers than in controls. The tail moments in the two groups were 1.75±0.29 and 1.47±0.41, respectively (P<0.0006). DNA damage of B-lymphocytes in the two groups showed the most significant difference among the three cell types. The tail moments were 3.86±0.71 and 1.51±0.39, respectively (P<0.0001). In granulocytes, DNA damage was also different, the tail moments being 3.61±0.75 and 2.60±0.59, respectively (P<0.0001). The comparison of DNA damage in both groups shows that B-lymphocytes could be a useful target in biomonitoring of human exposure to low levels of benzene.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalToxicology Letters
Volume134
Issue number1-3
DOIs
Publication statusPublished - 2002 Aug 5

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Keywords

  • Comet assay
  • Creatinine
  • Lymphocytes
  • t,t-MA

ASJC Scopus subject areas

  • Toxicology

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