Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli

William A. Rosche, Adam Jaworski, Seong Man Kang, Shannon F. Kramer, Jacquelynn E. Larson, David P. Geidroc, Robert D. Wells, Richard R. Sinden

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The stability of CTG triplet repeats was analyzed in Escherichia coli to identify processes responsible for their genetic instability. Using a biochemical assay for stability, we show that the absence of single-stranded- DNA-binding protein leads to an increase in the frequency of large deletions within the triplet repeats.

Original languageEnglish
Pages (from-to)5042-5044
Number of pages3
JournalJournal of Bacteriology
Volume178
Issue number16
Publication statusPublished - 1996 Oct 7
Externally publishedYes

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Trinucleotide Repeats
Protein Stability
DNA-Binding Proteins
Escherichia coli

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Rosche, W. A., Jaworski, A., Kang, S. M., Kramer, S. F., Larson, J. E., Geidroc, D. P., ... Sinden, R. R. (1996). Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli. Journal of Bacteriology, 178(16), 5042-5044.

Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli. / Rosche, William A.; Jaworski, Adam; Kang, Seong Man; Kramer, Shannon F.; Larson, Jacquelynn E.; Geidroc, David P.; Wells, Robert D.; Sinden, Richard R.

In: Journal of Bacteriology, Vol. 178, No. 16, 07.10.1996, p. 5042-5044.

Research output: Contribution to journalArticle

Rosche, WA, Jaworski, A, Kang, SM, Kramer, SF, Larson, JE, Geidroc, DP, Wells, RD & Sinden, RR 1996, 'Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli', Journal of Bacteriology, vol. 178, no. 16, pp. 5042-5044.
Rosche WA, Jaworski A, Kang SM, Kramer SF, Larson JE, Geidroc DP et al. Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli. Journal of Bacteriology. 1996 Oct 7;178(16):5042-5044.
Rosche, William A. ; Jaworski, Adam ; Kang, Seong Man ; Kramer, Shannon F. ; Larson, Jacquelynn E. ; Geidroc, David P. ; Wells, Robert D. ; Sinden, Richard R. / Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli. In: Journal of Bacteriology. 1996 ; Vol. 178, No. 16. pp. 5042-5044.
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