Single wall carbon nanotube electrode system capable of quantitative detection of CD4+ T cells

Joonhyub Kim, Gayoung Park, Seoho Lee, Suk-Won Hwang, Namki Min, Kyung-Mi Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Development of CNT-based CD4+ T cell imunosensors remains in its infancy due to the poor immobilization efficiency, lack of reproducibility, and difficulty in providing linear quantification. Here, we developed a fully-integrated single wall carbon nanotube (SWCNT)-based immunosensor capable of selective capture and linear quantification of CD4+ T cells with greater dynamic range. By employing repeated two-step oxygen (O2) plasma treatment processes with 35 days of recovery periods, we achieved the enhanced functionalization of the CNT surface and the removal of the byproduct of spray-coated SWCNTs that hinders charge transfer and stable CD4+ T cell sensing. As a result, a linear electrochemical signal was generated in direct proportion to the bound cells. The slope of a SWCNT electrode in a target concentration range (102~106 cells/mL) was 4.55×10−2 μA per concentration decade, with the lowest detection limit of 1×102 cells/mL. Since the reduced number of CD4+ T cell counts in patients’ peripheral blood corresponds to the progression of HIV disease, our CD4+ T cell-immunosensor provides a simple and low-cost platform which can fulfill the requirement for the development of point-of-care (POC) diagnostic technologies for human immunodeficiency virus (HIV) patients in resource-limited countries.

Original languageEnglish
Pages (from-to)238-244
Number of pages7
JournalBiosensors and Bioelectronics
Volume90
DOIs
Publication statusPublished - 2017 Apr 15

Keywords

  • CD4 T cells
  • Electrochemical analysis
  • O plasma treatment
  • SWCNT
  • T cell immunosensor

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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