SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization

Ashok Kumar, Gi Dae Park, Sanjay K.S. Patel, Sanath Kondaveeti, Sachin Otari, Muhammad Zahid Anwar, Vipin C. Kalia, Yogendra Singh, Sun Chang Kim, Byung Kwan Cho, Jung Hoon Sohn, Dong Rip Kim, Yun Chan Kang, Jung Kul Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Novel mesoporous SiO2 microparticles were synthesized by spray pyrolysis using multiwalled carbon nanotubes (MCNTs) as a template. The synthesized multicompartment structure with uniform pores of 12.0 nm was used to immobilize lipase from Thermomyces lanuginosus. The total surface area of mesoporous SiO2 microparticles prepared from silica colloidal solution was increased by 26-folds compared to that of dense SiO2 particles (494 vs 19.0 m2 g−1, respectively). Mesoporous SiO2 particles showed 236% higher protein loading for lipase, than dense SiO2 particles. The maximum velocity (Vmax) and catalytic efficiencies of immobilized lipase were 3.80 and 5.90 folds higher than that of free enzyme. Contact angle analysis revealed increased hydrophobicity of the mesoporous particles, which is advantageous for lid opening at the active center, and increased activity after immobilization. We next developed a lipase/SiO2/glassy carbon electrode (GCE) biosensors. Cyclic voltammetric results showed linear responses of the lipase/SiO2/GCE bioelectrode towards tributyrin (50–300 mg dL−1) as a surface-limited reaction in Tris-HCl buffer. After 12 repetitive uses, dense SiO2- and mesoporous SiO2-bound lipase retained 74.2 and 95.4% of its original activities, respectively. Thus, given their desirable characteristics and industrial utility, greatly porous SiO2 particles may provide an excellent support for enzyme immobilization in biosensor development or biocatalysis in organic media.

Original languageEnglish
Pages (from-to)1252-1264
Number of pages13
JournalChemical Engineering Journal
Volume359
DOIs
Publication statusPublished - 2019 Mar 1

Fingerprint

Enzyme immobilization
Carbon Nanotubes
Lipases
Lipase
immobilization
Carbon nanotubes
enzyme
Glassy carbon
electrode
Biosensors
fold
carbon
hydrophobicity
Electrodes
Spray pyrolysis
Multiwalled carbon nanotubes (MWCN)
pyrolysis
spray
Hydrophobicity
Silicon Dioxide

Keywords

  • Biosensor
  • Enzyme immobilization
  • Hydrophobicity
  • Mesoporous silica
  • Stability

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Kumar, A., Park, G. D., Patel, S. K. S., Kondaveeti, S., Otari, S., Anwar, M. Z., ... Lee, J. K. (2019). SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization. Chemical Engineering Journal, 359, 1252-1264. https://doi.org/10.1016/j.cej.2018.11.052

SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization. / Kumar, Ashok; Park, Gi Dae; Patel, Sanjay K.S.; Kondaveeti, Sanath; Otari, Sachin; Anwar, Muhammad Zahid; Kalia, Vipin C.; Singh, Yogendra; Kim, Sun Chang; Cho, Byung Kwan; Sohn, Jung Hoon; Kim, Dong Rip; Kang, Yun Chan; Lee, Jung Kul.

In: Chemical Engineering Journal, Vol. 359, 01.03.2019, p. 1252-1264.

Research output: Contribution to journalArticle

Kumar, A, Park, GD, Patel, SKS, Kondaveeti, S, Otari, S, Anwar, MZ, Kalia, VC, Singh, Y, Kim, SC, Cho, BK, Sohn, JH, Kim, DR, Kang, YC & Lee, JK 2019, 'SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization', Chemical Engineering Journal, vol. 359, pp. 1252-1264. https://doi.org/10.1016/j.cej.2018.11.052
Kumar, Ashok ; Park, Gi Dae ; Patel, Sanjay K.S. ; Kondaveeti, Sanath ; Otari, Sachin ; Anwar, Muhammad Zahid ; Kalia, Vipin C. ; Singh, Yogendra ; Kim, Sun Chang ; Cho, Byung Kwan ; Sohn, Jung Hoon ; Kim, Dong Rip ; Kang, Yun Chan ; Lee, Jung Kul. / SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization. In: Chemical Engineering Journal. 2019 ; Vol. 359. pp. 1252-1264.
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AU - Patel, Sanjay K.S.

AU - Kondaveeti, Sanath

AU - Otari, Sachin

AU - Anwar, Muhammad Zahid

AU - Kalia, Vipin C.

AU - Singh, Yogendra

AU - Kim, Sun Chang

AU - Cho, Byung Kwan

AU - Sohn, Jung Hoon

AU - Kim, Dong Rip

AU - Kang, Yun Chan

AU - Lee, Jung Kul

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N2 - Novel mesoporous SiO2 microparticles were synthesized by spray pyrolysis using multiwalled carbon nanotubes (MCNTs) as a template. The synthesized multicompartment structure with uniform pores of 12.0 nm was used to immobilize lipase from Thermomyces lanuginosus. The total surface area of mesoporous SiO2 microparticles prepared from silica colloidal solution was increased by 26-folds compared to that of dense SiO2 particles (494 vs 19.0 m2 g−1, respectively). Mesoporous SiO2 particles showed 236% higher protein loading for lipase, than dense SiO2 particles. The maximum velocity (Vmax) and catalytic efficiencies of immobilized lipase were 3.80 and 5.90 folds higher than that of free enzyme. Contact angle analysis revealed increased hydrophobicity of the mesoporous particles, which is advantageous for lid opening at the active center, and increased activity after immobilization. We next developed a lipase/SiO2/glassy carbon electrode (GCE) biosensors. Cyclic voltammetric results showed linear responses of the lipase/SiO2/GCE bioelectrode towards tributyrin (50–300 mg dL−1) as a surface-limited reaction in Tris-HCl buffer. After 12 repetitive uses, dense SiO2- and mesoporous SiO2-bound lipase retained 74.2 and 95.4% of its original activities, respectively. Thus, given their desirable characteristics and industrial utility, greatly porous SiO2 particles may provide an excellent support for enzyme immobilization in biosensor development or biocatalysis in organic media.

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