SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization

Ashok Kumar, Gi Dae Park, Sanjay K.S. Patel, Sanath Kondaveeti, Sachin Otari, Muhammad Zahid Anwar, Vipin C. Kalia, Yogendra Singh, Sun Chang Kim, Byung Kwan Cho, Jung Hoon Sohn, Dong Rip Kim, Yun Chan Kang, Jung Kul Lee

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Abstract

Novel mesoporous SiO2 microparticles were synthesized by spray pyrolysis using multiwalled carbon nanotubes (MCNTs) as a template. The synthesized multicompartment structure with uniform pores of 12.0 nm was used to immobilize lipase from Thermomyces lanuginosus. The total surface area of mesoporous SiO2 microparticles prepared from silica colloidal solution was increased by 26-folds compared to that of dense SiO2 particles (494 vs 19.0 m2 g−1, respectively). Mesoporous SiO2 particles showed 236% higher protein loading for lipase, than dense SiO2 particles. The maximum velocity (Vmax) and catalytic efficiencies of immobilized lipase were 3.80 and 5.90 folds higher than that of free enzyme. Contact angle analysis revealed increased hydrophobicity of the mesoporous particles, which is advantageous for lid opening at the active center, and increased activity after immobilization. We next developed a lipase/SiO2/glassy carbon electrode (GCE) biosensors. Cyclic voltammetric results showed linear responses of the lipase/SiO2/GCE bioelectrode towards tributyrin (50–300 mg dL−1) as a surface-limited reaction in Tris-HCl buffer. After 12 repetitive uses, dense SiO2- and mesoporous SiO2-bound lipase retained 74.2 and 95.4% of its original activities, respectively. Thus, given their desirable characteristics and industrial utility, greatly porous SiO2 particles may provide an excellent support for enzyme immobilization in biosensor development or biocatalysis in organic media.

Original languageEnglish
Pages (from-to)1252-1264
Number of pages13
JournalChemical Engineering Journal
Volume359
DOIs
Publication statusPublished - 2019 Mar 1

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Keywords

  • Biosensor
  • Enzyme immobilization
  • Hydrophobicity
  • Mesoporous silica
  • Stability

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Kumar, A., Park, G. D., Patel, S. K. S., Kondaveeti, S., Otari, S., Anwar, M. Z., Kalia, V. C., Singh, Y., Kim, S. C., Cho, B. K., Sohn, J. H., Kim, D. R., Kang, Y. C., & Lee, J. K. (2019). SiO2 microparticles with carbon nanotube-derived mesopores as an efficient support for enzyme immobilization. Chemical Engineering Journal, 359, 1252-1264. https://doi.org/10.1016/j.cej.2018.11.052