Abstract
Precise control of media delivery to cells in microfluidic systems in a simple and efficient manner is a challenge for a number of cell-based applications. Conventional syringe pumps can deliver culture media into microfluidic devices at precisely controlled flow rates, but they are bulky and require a power source. On the other hand, passive microflow-generating systems cannot maintain continuous, controllable and long-term delivery of media. We have developed an on-chip microflow control technology that combines flow rate control and passive, long-term delivery of media to microwell tissue culture chambers. Here, a passive flow is initiated using the siphon effect and a yarn flow resistor is used to regulate the flow rate in the microchannel. Using the yarn flow resistor, the medium flow rate into the microfluidic cell culture system is made adjustable to a few hundred microliters per hour. To evaluate the effects of controlled flow on microfluidic cell culture properties (feasibility test), we measured the cell alignment and cytoskeletal arrangement of endothelial cells cultured in a microwell array inside the microfluidic channel. This journal is
| Original language | English |
|---|---|
| Pages (from-to) | 4213-4219 |
| Number of pages | 7 |
| Journal | Lab on a Chip - Miniaturisation for Chemistry and Biology |
| Volume | 14 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - 2014 Nov 7 |
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ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
- Bioengineering
- Biomedical Engineering
Cite this
Siphon-driven microfluidic passive pump with a yarn flow resistance controller. / Jeong, Gi Seok; Oh, Jonghyun; Kim, Sang Bok; Dokmeci, Mehmet Remzi; Bae, Hojae; Lee, Sang Hoon; Khademhosseini, Ali.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 14, No. 21, 07.11.2014, p. 4213-4219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Siphon-driven microfluidic passive pump with a yarn flow resistance controller
AU - Jeong, Gi Seok
AU - Oh, Jonghyun
AU - Kim, Sang Bok
AU - Dokmeci, Mehmet Remzi
AU - Bae, Hojae
AU - Lee, Sang Hoon
AU - Khademhosseini, Ali
PY - 2014/11/7
Y1 - 2014/11/7
N2 - Precise control of media delivery to cells in microfluidic systems in a simple and efficient manner is a challenge for a number of cell-based applications. Conventional syringe pumps can deliver culture media into microfluidic devices at precisely controlled flow rates, but they are bulky and require a power source. On the other hand, passive microflow-generating systems cannot maintain continuous, controllable and long-term delivery of media. We have developed an on-chip microflow control technology that combines flow rate control and passive, long-term delivery of media to microwell tissue culture chambers. Here, a passive flow is initiated using the siphon effect and a yarn flow resistor is used to regulate the flow rate in the microchannel. Using the yarn flow resistor, the medium flow rate into the microfluidic cell culture system is made adjustable to a few hundred microliters per hour. To evaluate the effects of controlled flow on microfluidic cell culture properties (feasibility test), we measured the cell alignment and cytoskeletal arrangement of endothelial cells cultured in a microwell array inside the microfluidic channel. This journal is
AB - Precise control of media delivery to cells in microfluidic systems in a simple and efficient manner is a challenge for a number of cell-based applications. Conventional syringe pumps can deliver culture media into microfluidic devices at precisely controlled flow rates, but they are bulky and require a power source. On the other hand, passive microflow-generating systems cannot maintain continuous, controllable and long-term delivery of media. We have developed an on-chip microflow control technology that combines flow rate control and passive, long-term delivery of media to microwell tissue culture chambers. Here, a passive flow is initiated using the siphon effect and a yarn flow resistor is used to regulate the flow rate in the microchannel. Using the yarn flow resistor, the medium flow rate into the microfluidic cell culture system is made adjustable to a few hundred microliters per hour. To evaluate the effects of controlled flow on microfluidic cell culture properties (feasibility test), we measured the cell alignment and cytoskeletal arrangement of endothelial cells cultured in a microwell array inside the microfluidic channel. This journal is
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U2 - 10.1039/c4lc00510d
DO - 10.1039/c4lc00510d
M3 - Article
VL - 14
SP - 4213
EP - 4219
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 21
ER -