SIRT1 interacts with and protects glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from nuclear translocation: Implications for cell survival after irradiation

Hyun Yoo Joo, Seon Rang Woo, Yan Nan Shen, Mi Yong Yun, Hyun Jin Shin, Eun Ran Park, Su Hyeon Kim, Jeong Eun Park, Yeun Jin Ju, Sung Hee Hong, Sang Gu Hwang, Myung Haing Cho, Joon Kim, Kee Ho Lee

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18 Citations (Scopus)

Abstract

Upon apoptotic stimulation, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cytosolic enzyme normally active in glycolysis, translocates into the nucleus and activates an apoptotic cascade therein. In the present work, we show that SIRT1 prevents nuclear translocation of GAPDH via interaction with GAPDH. SIRT1 depletion triggered nuclear translocation of cytosolic GAPDH even in the absence of apoptotic stress. Such translocation was not, however, observed when SIRT1 enzymatic activity was inhibited, indicating that SIRT1 protein per se, rather than the deacetylase activity of the protein, is required to inhibit GAPDH translocation. Upon irradiation, SIRT1 prevented irradiation-induced nuclear translocation of GAPDH, accompanied by interaction of SIRT1 and GAPDH. Thus, SIRT1 functions to retain GAPDH in the cytosol, protecting the enzyme from nuclear translocation via interaction with these two proteins. This serves as a mechanism whereby SIRT1 regulates cell survival upon induction of apoptotic stress by means that include irradiation.

Original languageEnglish
Pages (from-to)681-686
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume424
Issue number4
DOIs
Publication statusPublished - 2012 Aug 10

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Keywords

  • GAPDH
  • Interaction
  • Irradiation
  • Nuclear translocation
  • SIRT1
  • Survival

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Joo, H. Y., Woo, S. R., Shen, Y. N., Yun, M. Y., Shin, H. J., Park, E. R., Kim, S. H., Park, J. E., Ju, Y. J., Hong, S. H., Hwang, S. G., Cho, M. H., Kim, J., & Lee, K. H. (2012). SIRT1 interacts with and protects glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from nuclear translocation: Implications for cell survival after irradiation. Biochemical and Biophysical Research Communications, 424(4), 681-686. https://doi.org/10.1016/j.bbrc.2012.07.006