Sirt1 Regulates Microtubule Dynamics Through Negative Regulation of Plk1 in Mitosis

Jin Ju Kim, Na Yeon Gil, Xiang Hua Zhang, Kwang Hoon Chun, Guowei Fang, Joon Kim, Hyeseong Cho, Chang Young Jang, Hyuk Jin Cha

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Although loss of Sirt1 leads to chromosome aneuploidy, which accounts for higher tumor susceptibility, the molecular mechanisms remain unclear. Herein, we demonstrate that Sirt1 directly regulates Plk1, of which activity is critical for mitotic progression and spindle dynamics. Depletion or inhibition of Sirt1 significantly perturbs the formation of the mitotic spindle, leading to defective chromosome segregation. Elevated depolymerization of the mitotic spindle following loss of Sirt1 was associated with the deregulation of Plk1 activity. Thus, we conclude that Sirt1 may contribute to a mitotic regulator that controls spindle dynamics through Plk1 activity, resulting in fine-tuning of Plk1 dependent microtubule dynamics. J. Cell. Biochem. 116: 1888-1897, 2015.

Original languageEnglish
Pages (from-to)1888-1897
Number of pages10
JournalJournal of Cellular Biochemistry
Volume116
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

Keywords

  • MICROTUBULE DYNAMICS
  • MITOTIC ARREST
  • NOCODAZOLE
  • Plk1
  • Sirt1

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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