Site-directed mutation effect of the symmetry region at the mRNA 5′-end of Escherichia coli aeg-46.5 gene

JuHyuk Ahn, MuHyeon Choe

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1 Citation (Scopus)

Abstract

The age-46.5 gene of Escherichia coli is induced by nitrate ion and regulated by Fnr, NarL, and NarP during anaerobic growth. aeg-46.5::lacZ fusion gene shows its maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Fnr and NarP act as positive regulators, and NarL acts as a negative regulator. The control region of the aeg-46.5 was identified and the binding sites of regulator proteins have been predicted (Reznikoff and Choe (1993)). It has two symmetry regions. One is located at -52∼-37 bp from the anaerobic mRNA 5′-end, which is the binding site of NarL and NarP. The other is located at +37∼+56 bp from the 5′-end of mRNA. In this study, the downstream symmetry region from the mRNA 5′-end was investigated by site-directed mutagenesis. The destruction of the symmetry region increases the expression level of aeg-46.5. We propose that the symmetry region interferes with the expression of aeg-46.5 possibly by forming a stem-and-loop structure.

Original languageEnglish
Pages (from-to)92-97
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume29
Issue number1
Publication statusPublished - 1996 Jan 31

Fingerprint

Escherichia coli
Genes
Nitrates
Messenger RNA
Mutation
Binding Sites
Mutagenesis
Lac Operon
Site-Directed Mutagenesis
Fusion reactions
Switches
Ions
Growth
Proteins

Keywords

  • aeg-46.5
  • Anaerobically expressed gene
  • NarL
  • Site-directed mutagenesis
  • Symmetry region

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

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abstract = "The age-46.5 gene of Escherichia coli is induced by nitrate ion and regulated by Fnr, NarL, and NarP during anaerobic growth. aeg-46.5::lacZ fusion gene shows its maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Fnr and NarP act as positive regulators, and NarL acts as a negative regulator. The control region of the aeg-46.5 was identified and the binding sites of regulator proteins have been predicted (Reznikoff and Choe (1993)). It has two symmetry regions. One is located at -52∼-37 bp from the anaerobic mRNA 5′-end, which is the binding site of NarL and NarP. The other is located at +37∼+56 bp from the 5′-end of mRNA. In this study, the downstream symmetry region from the mRNA 5′-end was investigated by site-directed mutagenesis. The destruction of the symmetry region increases the expression level of aeg-46.5. We propose that the symmetry region interferes with the expression of aeg-46.5 possibly by forming a stem-and-loop structure.",
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AU - Ahn, JuHyuk

AU - Choe, MuHyeon

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N2 - The age-46.5 gene of Escherichia coli is induced by nitrate ion and regulated by Fnr, NarL, and NarP during anaerobic growth. aeg-46.5::lacZ fusion gene shows its maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Fnr and NarP act as positive regulators, and NarL acts as a negative regulator. The control region of the aeg-46.5 was identified and the binding sites of regulator proteins have been predicted (Reznikoff and Choe (1993)). It has two symmetry regions. One is located at -52∼-37 bp from the anaerobic mRNA 5′-end, which is the binding site of NarL and NarP. The other is located at +37∼+56 bp from the 5′-end of mRNA. In this study, the downstream symmetry region from the mRNA 5′-end was investigated by site-directed mutagenesis. The destruction of the symmetry region increases the expression level of aeg-46.5. We propose that the symmetry region interferes with the expression of aeg-46.5 possibly by forming a stem-and-loop structure.

AB - The age-46.5 gene of Escherichia coli is induced by nitrate ion and regulated by Fnr, NarL, and NarP during anaerobic growth. aeg-46.5::lacZ fusion gene shows its maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Fnr and NarP act as positive regulators, and NarL acts as a negative regulator. The control region of the aeg-46.5 was identified and the binding sites of regulator proteins have been predicted (Reznikoff and Choe (1993)). It has two symmetry regions. One is located at -52∼-37 bp from the anaerobic mRNA 5′-end, which is the binding site of NarL and NarP. The other is located at +37∼+56 bp from the 5′-end of mRNA. In this study, the downstream symmetry region from the mRNA 5′-end was investigated by site-directed mutagenesis. The destruction of the symmetry region increases the expression level of aeg-46.5. We propose that the symmetry region interferes with the expression of aeg-46.5 possibly by forming a stem-and-loop structure.

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