Site-specific incorporation of μ-N-crotonyllysine into histones

Chan Hyuk Kim, Mingchao Kang, Hak Joong Kim, Abhishek Chatterjee, Peter G. Schultz

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.

Original languageEnglish
Pages (from-to)7246-7249
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number29
DOIs
Publication statusPublished - 2012 Jul 16

Keywords

  • Crotonylations
  • Histones
  • Nonnatural amino acids
  • Post-translational modifications

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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