SK&F 96365 induces apoptosis and autophagy by inhibiting Akt-mTOR signaling in A7r5 cells

Eun Jung Park, Sang Yeob Kim, Su Hwa Kim, Chae Ryun Lee, In San Kim, Jong Kwan Park, Sung Won Lee, Byung Joo Kim, Jung Nyeo Chun, Insuk So, Ju Hong Jeon

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

SK&F 96365 has been widely used as an inhibitor of transient receptor potential (TRP) calcium channels in various physiological settings. However, growing evidence suggests that SK&F 96365 affects several cellular and molecular processes via uncharacterized off-target mechanisms. In this study, we showed that SK&F 96365 induces apoptosis and autophagy in A7r5 vascular smooth muscle cells. The combined suppression of apoptosis and autophagy provoked necrosis rather than rescued cell death in the cells treated with SK&F 96365. In addition, we found that SK&F 96365 inhibits Akt-mTOR signaling pathways, which is comparable with the efficacy of other known Akt inhibitors. Our findings suggest that SK&F 96365 can be a useful agent for delineating the molecular mechanisms underlying crosstalk among cell death pathways.

Original languageEnglish
Pages (from-to)2157-2164
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1813
Issue number12
DOIs
Publication statusPublished - 2011 Dec
Externally publishedYes

Keywords

  • Akt-mTOR signaling
  • Apoptosis
  • Autophagy
  • SK&F 96365
  • TRP channel

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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  • Cite this

    Park, E. J., Kim, S. Y., Kim, S. H., Lee, C. R., Kim, I. S., Park, J. K., Lee, S. W., Kim, B. J., Chun, J. N., So, I., & Jeon, J. H. (2011). SK&F 96365 induces apoptosis and autophagy by inhibiting Akt-mTOR signaling in A7r5 cells. Biochimica et Biophysica Acta - Molecular Cell Research, 1813(12), 2157-2164. https://doi.org/10.1016/j.bbamcr.2011.06.021