Small-animal PET imaging analysis with [18F]FHBG in a mouse model of HSV1-tk gene expression in melanoma

Onseok Lee, Jaeyoung Kim, Chil Hwan Oh

Research output: Contribution to journalLetter

Abstract

The purpose of this study was to establish a small-animal model for molecular imaging and to acquire basic data on assessing the efficacy of candidate melanoma drugs using small-animal PET imaging analysis with [18F]FHBG for herpes simplex virus 1-thymidine kinase (HSV1-tk) gene expression in a melanoma mouse model. The B16 melanoma cell line was transduced with a recombinant lentiviral vector containing the HSV1-tk gene and inoculated into the back skin of C57BL/6J mice. [18F]FHBG PET imaging showed better contrast for HSV1-tk(+) melanomas compared to brain, heart, gall bladder, intestine and kidney than did [18F]FDG PET imaging.

Original languageEnglish
Pages (from-to)199-201
Number of pages3
JournalExperimental Dermatology
Volume27
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

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Thymidine Kinase
Human Herpesvirus 1
Viruses
Gene expression
Melanoma
Animals
Gene Expression
Imaging techniques
Molecular imaging
Experimental Melanomas
Molecular Imaging
Fluorodeoxyglucose F18
Inbred C57BL Mouse
Intestines
Brain
Skin
Urinary Bladder
Animal Models
Genes
Cells

Keywords

  • HSV1-tk
  • lentiviral vector
  • melanoma
  • small-animal PET/CT
  • [F]FDG
  • [F]FHBG

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Small-animal PET imaging analysis with [18F]FHBG in a mouse model of HSV1-tk gene expression in melanoma. / Lee, Onseok; Kim, Jaeyoung; Oh, Chil Hwan.

In: Experimental Dermatology, Vol. 27, No. 2, 01.02.2018, p. 199-201.

Research output: Contribution to journalLetter

Lee, Onseok ; Kim, Jaeyoung ; Oh, Chil Hwan. / Small-animal PET imaging analysis with [18F]FHBG in a mouse model of HSV1-tk gene expression in melanoma. In: Experimental Dermatology. 2018 ; Vol. 27, No. 2. pp. 199-201.
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