Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function

Nicholas Kwiatkowski, Nannette Jelluma, Panagis Filippakopoulos, Meera Soundararajan, Michael S. Manak, Mijung Kwon, Hwan Geun Choi, Taebo Sim, Quinn L. Deveraux, Sabine Rottmann, David Pellman, Jagesh V. Shah, Geert J P L Kops, Stefan Knapp, Nathanael S. Gray

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Mps1, a dual-specificity kinase, is required for the proper functioning of the spindle assembly checkpoint and for the maintenance of chromosomal stability. As Mps1 function has been implicated in numerous phases of the cell cycle, the development of a potent, selective small-molecule inhibitor of Mps1 should facilitate dissection of Mps1-related biology. We describe the cellular effects and Mps1 cocrystal structures of new, selective small-molecule inhibitors of Mps1. Consistent with RNAi studies, chemical inhibition of Mps1 leads to defects in Mad1 and Mad2 establishment at unattached kinetochores, decreased Aurora B kinase activity, premature mitotic exit and gross aneuploidy, without any evidence of centrosome duplication defects. However, in U2OS cells having extra centrosomes (an abnormality found in some cancers), Mps1 inhibition increases the frequency of multipolar mitoses. Lastly, Mps1 inhibitor treatment resulted in a decrease in cancer cell viability.

Original languageEnglish
Pages (from-to)359-368
Number of pages10
JournalNature Chemical Biology
Volume6
Issue number5
DOIs
Publication statusPublished - 2010 May 1
Externally publishedYes

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Centrosome
Cell Cycle
Phosphotransferases
Aurora Kinase B
M Phase Cell Cycle Checkpoints
Kinetochores
Chromosomal Instability
Aneuploidy
RNA Interference
Mitosis
Dissection
Neoplasms
Cell Survival
Maintenance

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Kwiatkowski, N., Jelluma, N., Filippakopoulos, P., Soundararajan, M., Manak, M. S., Kwon, M., ... Gray, N. S. (2010). Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function. Nature Chemical Biology, 6(5), 359-368. https://doi.org/10.1038/nchembio.345

Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function. / Kwiatkowski, Nicholas; Jelluma, Nannette; Filippakopoulos, Panagis; Soundararajan, Meera; Manak, Michael S.; Kwon, Mijung; Choi, Hwan Geun; Sim, Taebo; Deveraux, Quinn L.; Rottmann, Sabine; Pellman, David; Shah, Jagesh V.; Kops, Geert J P L; Knapp, Stefan; Gray, Nathanael S.

In: Nature Chemical Biology, Vol. 6, No. 5, 01.05.2010, p. 359-368.

Research output: Contribution to journalArticle

Kwiatkowski, N, Jelluma, N, Filippakopoulos, P, Soundararajan, M, Manak, MS, Kwon, M, Choi, HG, Sim, T, Deveraux, QL, Rottmann, S, Pellman, D, Shah, JV, Kops, GJPL, Knapp, S & Gray, NS 2010, 'Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function', Nature Chemical Biology, vol. 6, no. 5, pp. 359-368. https://doi.org/10.1038/nchembio.345
Kwiatkowski N, Jelluma N, Filippakopoulos P, Soundararajan M, Manak MS, Kwon M et al. Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function. Nature Chemical Biology. 2010 May 1;6(5):359-368. https://doi.org/10.1038/nchembio.345
Kwiatkowski, Nicholas ; Jelluma, Nannette ; Filippakopoulos, Panagis ; Soundararajan, Meera ; Manak, Michael S. ; Kwon, Mijung ; Choi, Hwan Geun ; Sim, Taebo ; Deveraux, Quinn L. ; Rottmann, Sabine ; Pellman, David ; Shah, Jagesh V. ; Kops, Geert J P L ; Knapp, Stefan ; Gray, Nathanael S. / Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function. In: Nature Chemical Biology. 2010 ; Vol. 6, No. 5. pp. 359-368.
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