Small-molecule probes elucidate global enzyme activity in a proteomic context

Jun Seok Lee, Young Hwa Yoo, Chang No Yoon

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The recent dramatic improvements in high-resolution mass spectrometry (MS) have revolutionized the speed and scope of proteomic studies. Conventional MS-based proteomics methodologies allow global protein profiling based on expression levels. Although these techniques are promising, there are numerous biological activities yet to be unveiled, such as the dynamic regulation of enzyme activity. Chemical proteomics is an emerging field that extends these types proteomic profiling. In particular, activity-based protein profiling (ABPP) utilizes small-molecule probes to monitor enzyme activity directly in living intact subjects. In this mini-review, we summarize the unique roles of smallmolecule probes in proteomics studies and highlight some recent examples in which this principle has been applied.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalBMB reports
Issue number3
Publication statusPublished - 2014 Mar
Externally publishedYes


  • Activity-based protein profiling
  • Fluorescent imaging
  • Proteomics
  • Small molecule probes
  • Systems biology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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