SMD and NMD are competitive pathways that contribute to myogenesis: Effects on PAX3 and myogenin mRNAs

Chenguang Gong, Yoon Ki Kim, Collynn F. Woeller, Yalan Tang, Lynne E. Maquat

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

UPF1 functions in both Staufen 1 (STAU1)-mediated mRNA decay (SMD) and nonsense-mediated mRNA decay (NMD), which we show here are competitive pathways. STAU1- and UPF2-binding sites within UPF1 overlap so that STAU1 and UPF2 binding to UPF1 appear to be mutually exclusive. Furthermore, down-regulating the cellular abundance of STAU1, which inhibits SMD, increases the efficiency of NMD, whereas down-regulating the cellular abundance of UPF2, which inhibits NMD, increases the efficiency of SMD. Competition under physiological conditions is exemplified during the differentiation of C2C12 myoblasts to myotubes: The efficiency of SMD increases and the efficiency of NMD decreases, consistent with our finding that more STAU1 but less UPF2 bind UPF1 in myotubes compared with myoblasts. Moreover, an increase in the cellular level of UPF3X during myogenesis results in an increase in the efficiency of an alternative NMD pathway that, unlike classical NMD, is largely insensitive to UPF2 down-regulation. We discuss the remarkable balance between SMD and the two types of NMD in view of data indicating that PAX3 mRNA is an SMD target whose decay promotes myogenesis whereas myogenin mRNA is a classical NMD target encoding a protein required for myogenesis.

Original languageEnglish
Pages (from-to)54-66
Number of pages13
JournalGenes and Development
Volume23
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Nonsense Mediated mRNA Decay
Myogenin
Muscle Development
RNA Stability
Messenger RNA
Myoblasts
Skeletal Muscle Fibers
Down-Regulation
Binding Sites

Keywords

  • Myogenesis
  • Nonsense-mediated mrna decay
  • Premature termination codon
  • Staufen1
  • Staufen1-mediated mrna decay
  • Upf proteins

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

SMD and NMD are competitive pathways that contribute to myogenesis : Effects on PAX3 and myogenin mRNAs. / Gong, Chenguang; Kim, Yoon Ki; Woeller, Collynn F.; Tang, Yalan; Maquat, Lynne E.

In: Genes and Development, Vol. 23, No. 1, 01.01.2009, p. 54-66.

Research output: Contribution to journalArticle

Gong, Chenguang ; Kim, Yoon Ki ; Woeller, Collynn F. ; Tang, Yalan ; Maquat, Lynne E. / SMD and NMD are competitive pathways that contribute to myogenesis : Effects on PAX3 and myogenin mRNAs. In: Genes and Development. 2009 ; Vol. 23, No. 1. pp. 54-66.
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