Smoking status-dependent association of the 27-bp repeat polymorphism in intron 4 of endothelial nitric oxide synthase gene with plasma nitric oxide concentrations

Suin Yoon, Jesung Moon, Chol Shin, Eunkyung Kim, Sangmee Ahn Jo, Inho Jo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background: Both positive and negative associations between a rare allele of 27-bp repeat polymorphism (eNOS4b/a polymorphism) in intron 4 of endothelial nitric oxide synthase and plasma nitric oxide (NO) concentrations were previously reported. Although these conflicting results were suggested to be partly accounted for smoking status of subjects, no further studies have been accomplished. Methods: We analyzed eNOS4b/a polymorphism in a group of 393 healthy Korean subjects and measured their plasma nitrite and nitrate (NOx) concentrations. NOx concentrations were measured by the Griess method and the genotypes of eNOS4b/a polymorphism determined by the banding pattern on gel electrophoresis. Results: The frequency of eNOS4a allele in this study was 11.6%. The plasma NOx concentrations (in μmol/l) in subjects with eNOS4a allele was found to be significantly higher relative to those in eNOS4b allele (49.68±18.62 and 55.25±20.87, respectively, P<0.05), which was valid only in smokers. Multiple regression analysis revealed that the most predictive contributing factor for plasma NOx concentrations was eNOS4a allele (P<0.01), followed by smoking (P<0.05), total cholesterol (P<0.05), and triglycerides (P<0.05). Conclusion: Our data indicate that there is substantial effect of eNOS4b/a polymorphism on the variance of plasma NOx concentrations in Korean population and that this effect is dependent on smoking status.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalClinica Chimica Acta
Volume324
Issue number1-2
DOIs
Publication statusPublished - 2002 Oct 1

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Nitric Oxide Synthase Type III
Polymorphism
Introns
Nitric Oxide
Genes
Smoking
Association reactions
Plasmas
Alleles
Nitrites
Electrophoresis
Gene Frequency
Regression analysis
Nitrates
Healthy Volunteers
Triglycerides
Gels
Cholesterol
Genotype
Regression Analysis

Keywords

  • Endothelial nitric oxide synthase gene
  • Korea
  • Plasma nitric oxide concentrations
  • Polymorphism
  • Smoking status

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Smoking status-dependent association of the 27-bp repeat polymorphism in intron 4 of endothelial nitric oxide synthase gene with plasma nitric oxide concentrations. / Yoon, Suin; Moon, Jesung; Shin, Chol; Kim, Eunkyung; Jo, Sangmee Ahn; Jo, Inho.

In: Clinica Chimica Acta, Vol. 324, No. 1-2, 01.10.2002, p. 113-120.

Research output: Contribution to journalArticle

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abstract = "Background: Both positive and negative associations between a rare allele of 27-bp repeat polymorphism (eNOS4b/a polymorphism) in intron 4 of endothelial nitric oxide synthase and plasma nitric oxide (NO) concentrations were previously reported. Although these conflicting results were suggested to be partly accounted for smoking status of subjects, no further studies have been accomplished. Methods: We analyzed eNOS4b/a polymorphism in a group of 393 healthy Korean subjects and measured their plasma nitrite and nitrate (NOx) concentrations. NOx concentrations were measured by the Griess method and the genotypes of eNOS4b/a polymorphism determined by the banding pattern on gel electrophoresis. Results: The frequency of eNOS4a allele in this study was 11.6{\%}. The plasma NOx concentrations (in μmol/l) in subjects with eNOS4a allele was found to be significantly higher relative to those in eNOS4b allele (49.68±18.62 and 55.25±20.87, respectively, P<0.05), which was valid only in smokers. Multiple regression analysis revealed that the most predictive contributing factor for plasma NOx concentrations was eNOS4a allele (P<0.01), followed by smoking (P<0.05), total cholesterol (P<0.05), and triglycerides (P<0.05). Conclusion: Our data indicate that there is substantial effect of eNOS4b/a polymorphism on the variance of plasma NOx concentrations in Korean population and that this effect is dependent on smoking status.",
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T1 - Smoking status-dependent association of the 27-bp repeat polymorphism in intron 4 of endothelial nitric oxide synthase gene with plasma nitric oxide concentrations

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AU - Jo, Sangmee Ahn

AU - Jo, Inho

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N2 - Background: Both positive and negative associations between a rare allele of 27-bp repeat polymorphism (eNOS4b/a polymorphism) in intron 4 of endothelial nitric oxide synthase and plasma nitric oxide (NO) concentrations were previously reported. Although these conflicting results were suggested to be partly accounted for smoking status of subjects, no further studies have been accomplished. Methods: We analyzed eNOS4b/a polymorphism in a group of 393 healthy Korean subjects and measured their plasma nitrite and nitrate (NOx) concentrations. NOx concentrations were measured by the Griess method and the genotypes of eNOS4b/a polymorphism determined by the banding pattern on gel electrophoresis. Results: The frequency of eNOS4a allele in this study was 11.6%. The plasma NOx concentrations (in μmol/l) in subjects with eNOS4a allele was found to be significantly higher relative to those in eNOS4b allele (49.68±18.62 and 55.25±20.87, respectively, P<0.05), which was valid only in smokers. Multiple regression analysis revealed that the most predictive contributing factor for plasma NOx concentrations was eNOS4a allele (P<0.01), followed by smoking (P<0.05), total cholesterol (P<0.05), and triglycerides (P<0.05). Conclusion: Our data indicate that there is substantial effect of eNOS4b/a polymorphism on the variance of plasma NOx concentrations in Korean population and that this effect is dependent on smoking status.

AB - Background: Both positive and negative associations between a rare allele of 27-bp repeat polymorphism (eNOS4b/a polymorphism) in intron 4 of endothelial nitric oxide synthase and plasma nitric oxide (NO) concentrations were previously reported. Although these conflicting results were suggested to be partly accounted for smoking status of subjects, no further studies have been accomplished. Methods: We analyzed eNOS4b/a polymorphism in a group of 393 healthy Korean subjects and measured their plasma nitrite and nitrate (NOx) concentrations. NOx concentrations were measured by the Griess method and the genotypes of eNOS4b/a polymorphism determined by the banding pattern on gel electrophoresis. Results: The frequency of eNOS4a allele in this study was 11.6%. The plasma NOx concentrations (in μmol/l) in subjects with eNOS4a allele was found to be significantly higher relative to those in eNOS4b allele (49.68±18.62 and 55.25±20.87, respectively, P<0.05), which was valid only in smokers. Multiple regression analysis revealed that the most predictive contributing factor for plasma NOx concentrations was eNOS4a allele (P<0.01), followed by smoking (P<0.05), total cholesterol (P<0.05), and triglycerides (P<0.05). Conclusion: Our data indicate that there is substantial effect of eNOS4b/a polymorphism on the variance of plasma NOx concentrations in Korean population and that this effect is dependent on smoking status.

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