SNX14 is a bifunctional negative regulator for neuronal 5-HT6 receptor signaling

Chang Man Ha, Daehun Park, Yoonju Kim, Myeongsu Na, Surabhi Panda, Sehoon Won, Hyun Kim, Hoon Ryu, Zee Yong Park, Mark M. Rasenick, Sunghoe Chang

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


The 5-hydroxytryptamine (5-HT, also known as serotonin) subtype 6 receptor (5-HT6R, also known as HTR6) plays roles in cognition, anxiety and learning and memory disorders, yet new details concerning its regulation remain poorly understood. In this study, we found that 5-HT6R directly interacted with SNX14 and that this interaction dramatically increased internalization and degradation of 5-HT6R. Knockdown of endogenous SNX14 had the opposite effect. SNX14 is highly expressed in the brain and contains a putative regulator of G-protein signaling (RGS) domain. Although its RGS domain was found to be non-functional as a GTPase activator for Gαs, we found that it specifically bound to and sequestered Gαs, thus inhibiting downstream cAMP production. We further found that protein kinase A (PKA)-mediated phosphorylation of SNX14 inhibited its binding to Gαs and diverted SNX14 from Gαs binding to 5-HT6R binding, thus facilitating the endocytic degradation of the receptor. Therefore, our results suggest that SNX14 is a dual endogenous negative regulator in 5-HT6R-mediated signaling pathway, modulating both signaling and trafficking of 5-HT6R.

Original languageEnglish
Pages (from-to)1848-1861
Number of pages14
JournalJournal of Cell Science
Issue number9
Publication statusPublished - 2015


  • 5-HT
  • 5-hydroxytryptamine type 6 receptor
  • G protein-coupled receptor
  • Guanosine triphosphataseactivating proteins
  • Regulator of G protein signaling
  • SNX14
  • Sorting nexin

ASJC Scopus subject areas

  • Cell Biology


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