Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice

Hsien Sung Huang, Bongjune Yoon, Sherian Brooks, Robert Bakal, Janet Berrios, Rylan S. Larsen, Michael L. Wallace, Ji Eun Han, Eui Hwan Chung, Mark J. Zylka, Benjamin D. Philpot

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Genomic imprinting describes an epigenetic process through which genes can be expressed in a parent-of-origin-specific manner. The monoallelic expression of imprinted genes renders them particularly susceptible to disease causing mutations. A large proportion of imprinted genes are expressed in the brain, but little is known about their functions. Indeed, it has proven difficult to identify cell type-specific imprinted genes due to the heterogeneity of cell types within the brain. Here we used laser capture microdissection of visual cortical neurons and found evidence that sorting nexin 14 (Snx14) is a neuronally imprinted gene in mice. SNX14 protein levels are high in the brain and progressively increase during neuronal development and maturation. Snx14 knockdown reduces intrinsic excitability and severely impairs both excitatory and inhibitory synaptic transmission. These data reveal a role for monoallelic Snx14 expression in maintaining normal neuronal excitability and synaptic transmission.

Original languageEnglish
Article numbere98383
JournalPLoS One
Volume9
Issue number5
DOIs
Publication statusPublished - 2014 May 23

Fingerprint

Sorting Nexins
synaptic transmission
Synaptic Transmission
sorting
Brain
Genes
brain
mice
genes
Genetic Epigenesis
Laser Capture Microdissection
Genomic Imprinting
Microdissection
genomic imprinting
biological resistance
epigenetics
lasers
neurons
Neurons
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice. / Huang, Hsien Sung; Yoon, Bongjune; Brooks, Sherian; Bakal, Robert; Berrios, Janet; Larsen, Rylan S.; Wallace, Michael L.; Han, Ji Eun; Chung, Eui Hwan; Zylka, Mark J.; Philpot, Benjamin D.

In: PLoS One, Vol. 9, No. 5, e98383, 23.05.2014.

Research output: Contribution to journalArticle

Huang, HS, Yoon, B, Brooks, S, Bakal, R, Berrios, J, Larsen, RS, Wallace, ML, Han, JE, Chung, EH, Zylka, MJ & Philpot, BD 2014, 'Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice', PLoS One, vol. 9, no. 5, e98383. https://doi.org/10.1371/journal.pone.0098383
Huang, Hsien Sung ; Yoon, Bongjune ; Brooks, Sherian ; Bakal, Robert ; Berrios, Janet ; Larsen, Rylan S. ; Wallace, Michael L. ; Han, Ji Eun ; Chung, Eui Hwan ; Zylka, Mark J. ; Philpot, Benjamin D. / Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice. In: PLoS One. 2014 ; Vol. 9, No. 5.
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