Solid-phase genotoxicity assay for organic compounds in soil

Renee R. Alexander, Namhyun Chung, Martin Alexander

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.

Original languageEnglish
Pages (from-to)420-425
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume18
Issue number3
DOIs
Publication statusPublished - 1999 Mar 1
Externally publishedYes

Fingerprint

genotoxicity
Organic compounds
organic compound
Assays
Soil
assay
Soils
Mutagens
soil
Pseudomonas putida
Poisons
Mutation Rate
Rifampin
mutation
Suspensions
Sediments
sediment
mutant
2-aminofluorene
mutagen

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Solid-phase genotoxicity assay for organic compounds in soil. / Alexander, Renee R.; Chung, Namhyun; Alexander, Martin.

In: Environmental Toxicology and Chemistry, Vol. 18, No. 3, 01.03.1999, p. 420-425.

Research output: Contribution to journalArticle

Alexander, Renee R. ; Chung, Namhyun ; Alexander, Martin. / Solid-phase genotoxicity assay for organic compounds in soil. In: Environmental Toxicology and Chemistry. 1999 ; Vol. 18, No. 3. pp. 420-425.
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