Solid-phase genotoxicity assay for organic compounds in soil

Renee R. Alexander, Namhyun Chung, Martin Alexander

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.

Original languageEnglish
Pages (from-to)420-425
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume18
Issue number3
DOIs
Publication statusPublished - 1999 Mar 1
Externally publishedYes

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ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

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