A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.
|Number of pages||6|
|Journal||Environmental Toxicology and Chemistry|
|Publication status||Published - 1999|
- Pseudomonas putida
ASJC Scopus subject areas
- Environmental Chemistry
- Health, Toxicology and Mutagenesis