Soluble overexpression and purification of bioactive human CCL2 in E. coli by maltose-binding protein

Thu Trang Thi Vu, Bon Kyung Koo, Jung A. Song, Seon Ha Chong, Cho Rong Park, Minh Tan Nguyen, Boram Jeong, Han Bong Ryu, Jae Young Seong, Yeon Jin Jang, Robert Charles Robinson, Han Choe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Human chemokine (C–C motif) ligand 2 (hCCL2) is a small cytokine in the CC chemokine family that attracts monocytes, memory T lymphocytes, and natural killer cells to the site of tissue injury- or infection-induced inflammation. hCCL2 has been implicated in the pathogeneses of diseases characterized by monocytic infiltrates, including psoriasis, rheumatoid arthritis, atherosclerosis, multiple sclerosis, and insulin-resistant diabetes. The prokaryotic overexpression of hCCL2 has been investigated previously in an attempt to develop biomedical applications for this factor, but this has been hampered by protein misfolding and aggregation into inclusion bodies. In our present study, we screened 7 protein tags—Trx, GST, MBP, NusA, His8, PDI, and PDIb′a′—for their ability to allow the soluble overexpression of hCCL2. Three tags—MBP, His8, and PDI—solubilized more than half of the expressed hCCL2 fusion proteins. Lowering the expression temperature to 18 °C significantly further improved the solubility of all fusion proteins. MBP was chosen for further study based on its solubility, expression level, ease of purification, and tag size. MBP-CCL2 was purified using conventional chromatography and cleaved using TEV or Factor Xa proteases. Biological activity was assessed using luciferase and cell migration assays. Factor Xa-cleaved hCCL2 was found to be active and TEV-cleaved hCCL2 showed relatively less activity. This is probably because the additional glycine residues present at the N-terminus of hCCL2 following TEV digestion interfere with the binding of hCCL2 to its receptor.

Original languageEnglish
Pages (from-to)651-663
Number of pages13
JournalMolecular Biology Reports
Volume42
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Maltose-Binding Proteins
CC Chemokines
Escherichia coli
Ligands
Factor Xa
Solubility
Proteins
Cell Migration Assays
Inclusion Bodies
Luciferases
Psoriasis
Natural Killer Cells
Glycine
Multiple Sclerosis
Chromatography
Monocytes
Digestion
Rheumatoid Arthritis
Atherosclerosis
Peptide Hydrolases

Keywords

  • Chemotatic migration assay
  • Human chemokine (C–C motif) ligand 2
  • Protein purification from E. coli
  • Soluble overexpression

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Vu, T. T. T., Koo, B. K., Song, J. A., Chong, S. H., Park, C. R., Nguyen, M. T., ... Choe, H. (2015). Soluble overexpression and purification of bioactive human CCL2 in E. coli by maltose-binding protein. Molecular Biology Reports, 42(3), 651-663. https://doi.org/10.1007/s11033-014-3812-3

Soluble overexpression and purification of bioactive human CCL2 in E. coli by maltose-binding protein. / Vu, Thu Trang Thi; Koo, Bon Kyung; Song, Jung A.; Chong, Seon Ha; Park, Cho Rong; Nguyen, Minh Tan; Jeong, Boram; Ryu, Han Bong; Seong, Jae Young; Jang, Yeon Jin; Robinson, Robert Charles; Choe, Han.

In: Molecular Biology Reports, Vol. 42, No. 3, 2015, p. 651-663.

Research output: Contribution to journalArticle

Vu, TTT, Koo, BK, Song, JA, Chong, SH, Park, CR, Nguyen, MT, Jeong, B, Ryu, HB, Seong, JY, Jang, YJ, Robinson, RC & Choe, H 2015, 'Soluble overexpression and purification of bioactive human CCL2 in E. coli by maltose-binding protein', Molecular Biology Reports, vol. 42, no. 3, pp. 651-663. https://doi.org/10.1007/s11033-014-3812-3
Vu, Thu Trang Thi ; Koo, Bon Kyung ; Song, Jung A. ; Chong, Seon Ha ; Park, Cho Rong ; Nguyen, Minh Tan ; Jeong, Boram ; Ryu, Han Bong ; Seong, Jae Young ; Jang, Yeon Jin ; Robinson, Robert Charles ; Choe, Han. / Soluble overexpression and purification of bioactive human CCL2 in E. coli by maltose-binding protein. In: Molecular Biology Reports. 2015 ; Vol. 42, No. 3. pp. 651-663.
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abstract = "Human chemokine (C–C motif) ligand 2 (hCCL2) is a small cytokine in the CC chemokine family that attracts monocytes, memory T lymphocytes, and natural killer cells to the site of tissue injury- or infection-induced inflammation. hCCL2 has been implicated in the pathogeneses of diseases characterized by monocytic infiltrates, including psoriasis, rheumatoid arthritis, atherosclerosis, multiple sclerosis, and insulin-resistant diabetes. The prokaryotic overexpression of hCCL2 has been investigated previously in an attempt to develop biomedical applications for this factor, but this has been hampered by protein misfolding and aggregation into inclusion bodies. In our present study, we screened 7 protein tags—Trx, GST, MBP, NusA, His8, PDI, and PDIb′a′—for their ability to allow the soluble overexpression of hCCL2. Three tags—MBP, His8, and PDI—solubilized more than half of the expressed hCCL2 fusion proteins. Lowering the expression temperature to 18 °C significantly further improved the solubility of all fusion proteins. MBP was chosen for further study based on its solubility, expression level, ease of purification, and tag size. MBP-CCL2 was purified using conventional chromatography and cleaved using TEV or Factor Xa proteases. Biological activity was assessed using luciferase and cell migration assays. Factor Xa-cleaved hCCL2 was found to be active and TEV-cleaved hCCL2 showed relatively less activity. This is probably because the additional glycine residues present at the N-terminus of hCCL2 following TEV digestion interfere with the binding of hCCL2 to its receptor.",
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AU - Nguyen, Minh Tan

AU - Jeong, Boram

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AU - Robinson, Robert Charles

AU - Choe, Han

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