Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction

Seon S. Kim, Hyun Jung Byun, Sang Hoon Kim, Han Hyoung Lee, Suk Jun Lee, Sang Joon Kim, Chung G. Park, Taehoon Chun

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4 Citations (Scopus)

Abstract

Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalBiotechnology Letters
Volume32
Issue number2
DOIs
Publication statusPublished - 2010 Jan 1

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Keywords

  • Co-receptor
  • Lymphocyte activation gene-3
  • Mixed lymphocyte reaction
  • Pig
  • T cell activation
  • Xenogeneic response

ASJC Scopus subject areas

  • Biotechnology

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