Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA

Inhong Kim, Mijeong Kang, Han Young Woo, Jin Woo Oh, Kwangseuk Kyhm

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-Acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 Å) and resonance energy transfer efficiency (EFRET = 58.7 %) of pure buffer solution become R0 = 38.6 Å and EFRET = 48.0 % when 80% DMSO/buffer mixture is added.

Original languageEnglish
Title of host publicationInternational Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015
EditorsDonghyun Kim, Min-Gon Kim, Seung-Han Park
PublisherSPIE
ISBN (Electronic)9781628416794
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes
Event2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 - Jeju, Korea, Republic of
Duration: 2015 Feb 252015 Feb 27

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9523
ISSN (Print)1605-7422

Conference

Conference2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015
CountryKorea, Republic of
CityJeju
Period15/2/2515/2/27

Fingerprint

Single-Stranded DNA
Energy Transfer
Conjugated polymers
Energy transfer
Polymers
DNA
Coloring Agents
Dyes
deoxyribonucleic acid
dyes
energy transfer
Organic solvents
Buffers
Dimethyl sulfoxide
buffers
polymers
decay
Dimethyl Sulfoxide
Fluorescence
fluorescence

Keywords

  • Conjugated polymer
  • Exciton
  • Fluorescence quenching
  • Forster resonance energy transfer (FRET)
  • Time-resolved fluorescence

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, I., Kang, M., Woo, H. Y., Oh, J. W., & Kyhm, K. (2015). Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. In D. Kim, M-G. Kim, & S-H. Park (Eds.), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015 [95230N] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9523). SPIE. https://doi.org/10.1117/12.2190332

Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. / Kim, Inhong; Kang, Mijeong; Woo, Han Young; Oh, Jin Woo; Kyhm, Kwangseuk.

International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015. ed. / Donghyun Kim; Min-Gon Kim; Seung-Han Park. SPIE, 2015. 95230N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9523).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kim, I, Kang, M, Woo, HY, Oh, JW & Kyhm, K 2015, Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. in D Kim, M-G Kim & S-H Park (eds), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015., 95230N, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9523, SPIE, 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015, Jeju, Korea, Republic of, 15/2/25. https://doi.org/10.1117/12.2190332
Kim I, Kang M, Woo HY, Oh JW, Kyhm K. Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. In Kim D, Kim M-G, Park S-H, editors, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015. SPIE. 2015. 95230N. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2190332
Kim, Inhong ; Kang, Mijeong ; Woo, Han Young ; Oh, Jin Woo ; Kyhm, Kwangseuk. / Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015. editor / Donghyun Kim ; Min-Gon Kim ; Seung-Han Park. SPIE, 2015. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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abstract = "We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-Acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 {\AA}) and resonance energy transfer efficiency (EFRET = 58.7 {\%}) of pure buffer solution become R0 = 38.6 {\AA} and EFRET = 48.0 {\%} when 80{\%} DMSO/buffer mixture is added.",
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