Some observations in freeze-drying of recombinant bioluminescent Escherichia coli for toxicity monitoring

Man Bock Gu, Sue Hyung Choi, Sung Woo Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A recombinant bioluminescent bacteria, containing a fabA::luxCDABE fusion gene, has been used to characterize freeze-drying methods, which may be conveniently used as a tool for the development of a portable biosensor. Through residual water, viability, biosensing activity and scanning electron microscopy analyses, the characteristics that four cryoprotectants, trehalose, sucrose, sorbitol, and mannitol, conferred on freeze-dried samples were elucidated, including the morphology, water content and activity of the cells. It was found that trehalose showed the best freeze-drying efficiency among the tested cryoprotectants and it might have a specific capacity limitation in protection of the cells during the freeze step. Humidity might result in damage to the cells, according to the viability, when exposed to air during storage, while the water remaining post freeze-drying showed good correlation with damage to the freeze-dried cells when under air-tight storage conditions. The results with other recombinant bioluminescent bacteria indicated that these findings might be general features of the freeze-drying processes.

Original languageEnglish
Pages (from-to)95-105
Number of pages11
JournalJournal of Biotechnology
Volume88
Issue number2
DOIs
Publication statusPublished - 2001 Jun 15
Externally publishedYes

Fingerprint

Freeze Drying
Escherichia coli
Trehalose
Water
Air
Bacteria
Cytoprotection
Sorbitol
Gene Fusion
Biosensing Techniques
Mannitol
Humidity
Electron Scanning Microscopy
Sucrose

Keywords

  • Freeze-drying
  • Portable biosensor
  • Recombinant bioluminescent bacteria

ASJC Scopus subject areas

  • Biotechnology

Cite this

Some observations in freeze-drying of recombinant bioluminescent Escherichia coli for toxicity monitoring. / Gu, Man Bock; Hyung Choi, Sue; Woo Kim, Sung.

In: Journal of Biotechnology, Vol. 88, No. 2, 15.06.2001, p. 95-105.

Research output: Contribution to journalArticle

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