Sound packing DNA: Packing open circular DNA with low-intensity ultrasound

Donghee Park, Bong Kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Yong Jun Jo, Jin Woo Chang, Sangwoo Lee, Daesung Yoon, Jongbum Seo, Chul Woo Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Supercoiling DNA (folding DNA into a more compact molecule) from open circular forms requires significant bending energy. The double helix is coiled into a higher order helix form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil form. Plasmid DNA subjected to 5.4 mW/cm 2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.

Original languageEnglish
Article number9846
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Apr 20

ASJC Scopus subject areas

  • General

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