Sound packing DNA

Packing open circular DNA with low-intensity ultrasound

Donghee Park, Bong Kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Yong Jun Jo, Jin Woo Chang, Sangwoo Lee, Dae Sung Yoon, Jongbum Seo, Chul Woo Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Supercoiling DNA (folding DNA into a more compact molecule) from open circular forms requires significant bending energy. The double helix is coiled into a higher order helix form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil form. Plasmid DNA subjected to 5.4 mW/cm 2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.

Original languageEnglish
Article number9846
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Apr 20

Fingerprint

Circular DNA
DNA
Genes
Plasmids
Radiation

ASJC Scopus subject areas

  • General

Cite this

Park, D., Jung, B. K., Park, H., Lee, H., Lee, G., Park, J., ... Kim, C. W. (2015). Sound packing DNA: Packing open circular DNA with low-intensity ultrasound. Scientific reports, 5, [9846]. https://doi.org/10.1038/srep09846

Sound packing DNA : Packing open circular DNA with low-intensity ultrasound. / Park, Donghee; Jung, Bong Kwang; Park, Hyunjin; Lee, Hyungbeen; Lee, Gyudo; Park, Jingam; Shin, Unchul; Won, Jong Ho; Jo, Yong Jun; Chang, Jin Woo; Lee, Sangwoo; Yoon, Dae Sung; Seo, Jongbum; Kim, Chul Woo.

In: Scientific reports, Vol. 5, 9846, 20.04.2015.

Research output: Contribution to journalArticle

Park, D, Jung, BK, Park, H, Lee, H, Lee, G, Park, J, Shin, U, Won, JH, Jo, YJ, Chang, JW, Lee, S, Yoon, DS, Seo, J & Kim, CW 2015, 'Sound packing DNA: Packing open circular DNA with low-intensity ultrasound', Scientific reports, vol. 5, 9846. https://doi.org/10.1038/srep09846
Park, Donghee ; Jung, Bong Kwang ; Park, Hyunjin ; Lee, Hyungbeen ; Lee, Gyudo ; Park, Jingam ; Shin, Unchul ; Won, Jong Ho ; Jo, Yong Jun ; Chang, Jin Woo ; Lee, Sangwoo ; Yoon, Dae Sung ; Seo, Jongbum ; Kim, Chul Woo. / Sound packing DNA : Packing open circular DNA with low-intensity ultrasound. In: Scientific reports. 2015 ; Vol. 5.
@article{b62813fadaab43c695119acfe54b250a,
title = "Sound packing DNA: Packing open circular DNA with low-intensity ultrasound",
abstract = "Supercoiling DNA (folding DNA into a more compact molecule) from open circular forms requires significant bending energy. The double helix is coiled into a higher order helix form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil form. Plasmid DNA subjected to 5.4 mW/cm 2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.",
author = "Donghee Park and Jung, {Bong Kwang} and Hyunjin Park and Hyungbeen Lee and Gyudo Lee and Jingam Park and Unchul Shin and Won, {Jong Ho} and Jo, {Yong Jun} and Chang, {Jin Woo} and Sangwoo Lee and Yoon, {Dae Sung} and Jongbum Seo and Kim, {Chul Woo}",
year = "2015",
month = "4",
day = "20",
doi = "10.1038/srep09846",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Sound packing DNA

T2 - Packing open circular DNA with low-intensity ultrasound

AU - Park, Donghee

AU - Jung, Bong Kwang

AU - Park, Hyunjin

AU - Lee, Hyungbeen

AU - Lee, Gyudo

AU - Park, Jingam

AU - Shin, Unchul

AU - Won, Jong Ho

AU - Jo, Yong Jun

AU - Chang, Jin Woo

AU - Lee, Sangwoo

AU - Yoon, Dae Sung

AU - Seo, Jongbum

AU - Kim, Chul Woo

PY - 2015/4/20

Y1 - 2015/4/20

N2 - Supercoiling DNA (folding DNA into a more compact molecule) from open circular forms requires significant bending energy. The double helix is coiled into a higher order helix form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil form. Plasmid DNA subjected to 5.4 mW/cm 2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.

AB - Supercoiling DNA (folding DNA into a more compact molecule) from open circular forms requires significant bending energy. The double helix is coiled into a higher order helix form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil form. Plasmid DNA subjected to 5.4 mW/cm 2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.

UR - http://www.scopus.com/inward/record.url?scp=84928141319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84928141319&partnerID=8YFLogxK

U2 - 10.1038/srep09846

DO - 10.1038/srep09846

M3 - Article

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 9846

ER -