Abstract
Intake of saturated and trans-fatty acids is a strong risk factor for coronary heart disease. We investigated the inhibitory effects of 2 hexane extracts from white (WBE) and black soybeans (BBE) on cellular fatty acid uptake in vitro. Transcellular uptake of elaidic acid (t18:1), a major trans-fatty acid present in processed foods, in Caco-2 monolayers was significantly reduced by 28.3 and 16.7% 60 min after WBE and BBE treatment, respectively. Results of flow cytometry (FACS) analysis showed significant reductions in boron-dipyrromethene (BODIPY) fluorescencelabeled fatty acid uptake by 35.4 and 40.2% with WBE and BBE treatment, respectively. BBE treatment significantly reduced the expression of fatty acid transport protein-4 and CD36 in Caco-2 cells, as determined by quantitative real time-polymerase chain reaction (qRT-PCR). Similar trends were found in WBE treatment, although to a lesser degree. These observations suggest that soybean extract may reduce fatty acid uptake and cellular fat accumulation by altering fatty acid transporter expression.
| Original language | English |
|---|---|
| Pages (from-to) | 237-242 |
| Number of pages | 6 |
| Journal | Food Science and Biotechnology |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2011 Feb |
Keywords
- CD-36
- Fatty acid transport protein-4
- Fatty acid uptake
- Soybean
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology