Soybean (Glycine max L. Merr.) hexane extracts inhibit cellular fatty acid uptake by reducing the expression of fatty acid transporters

Kyoungrok Do, Jungae Jeun, Soung Jin Houng, Hee Jin Jun, Ji Hae Lee, Yaoyao Jia, Sung-Joon Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Intake of saturated and trans-fatty acids is a strong risk factor for coronary heart disease. We investigated the inhibitory effects of 2 hexane extracts from white (WBE) and black soybeans (BBE) on cellular fatty acid uptake in vitro. Transcellular uptake of elaidic acid (t18:1), a major trans-fatty acid present in processed foods, in Caco-2 monolayers was significantly reduced by 28.3 and 16.7% 60 min after WBE and BBE treatment, respectively. Results of flow cytometry (FACS) analysis showed significant reductions in boron-dipyrromethene (BODIPY) fluorescencelabeled fatty acid uptake by 35.4 and 40.2% with WBE and BBE treatment, respectively. BBE treatment significantly reduced the expression of fatty acid transport protein-4 and CD36 in Caco-2 cells, as determined by quantitative real time-polymerase chain reaction (qRT-PCR). Similar trends were found in WBE treatment, although to a lesser degree. These observations suggest that soybean extract may reduce fatty acid uptake and cellular fat accumulation by altering fatty acid transporter expression.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalFood Science and Biotechnology
Volume20
Issue number1
DOIs
Publication statusPublished - 2011 Jul 4

Fingerprint

Hexanes
Soybeans
hexane
transporters
Glycine max
Fatty Acids
soybeans
fatty acids
uptake mechanisms
extracts
Trans Fatty Acids
trans fatty acids
Fatty Acid Transport Proteins
elaidic acid
Boron
Caco-2 Cells
transport proteins
processed foods
Therapeutics
boron

Keywords

  • CD-36
  • Fatty acid transport protein-4
  • Fatty acid uptake
  • Soybean

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Soybean (Glycine max L. Merr.) hexane extracts inhibit cellular fatty acid uptake by reducing the expression of fatty acid transporters. / Do, Kyoungrok; Jeun, Jungae; Houng, Soung Jin; Jun, Hee Jin; Lee, Ji Hae; Jia, Yaoyao; Lee, Sung-Joon.

In: Food Science and Biotechnology, Vol. 20, No. 1, 04.07.2011, p. 237-242.

Research output: Contribution to journalArticle

Do, Kyoungrok ; Jeun, Jungae ; Houng, Soung Jin ; Jun, Hee Jin ; Lee, Ji Hae ; Jia, Yaoyao ; Lee, Sung-Joon. / Soybean (Glycine max L. Merr.) hexane extracts inhibit cellular fatty acid uptake by reducing the expression of fatty acid transporters. In: Food Science and Biotechnology. 2011 ; Vol. 20, No. 1. pp. 237-242.
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