Spatiotemporal expression pattern of non-clustered protocadherin family members in the developing rat brain

S. Y. Kim, H. Sun Chung, Woong Sun, Hyun Kim

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Protocadherins (PCDHs) consist of the largest subgroup of the cadherin superfamily, and most PCDHs are expressed dominantly in the CNS. Because PCDHs are involved in the homophilic cell-cell adhesion, PCDHs in the nervous system have been suggested to play roles in the formation and maintenance of the synaptic connections. Although many PCDHs (>50) are in tandem arranged as a cluster in a specific chromosome locus, there are also considerable numbers of non-clustered PCDH members (∼20). In this study, we examined the spatiotemporal distribution of mRNAs for 12 non-clustered PCDHs in rat brain using in situ hybridization. Some of them (PCDH1, PCDH7, PCDH9, PCDH10, PCDH11, PCDH17, and PCDH20) exhibited region-dependent expression pattern in the cerebral cortex during the early postnatal stage (P3), which is a critical period for the establishment of specific synaptic connections: PCDH7 and PCDH20 mRNAs were predominantly expressed in the somatosensory (parietal) and visual (occipital) cortices, whereas PCDH11 and PCDH17 mRNAs were preferentially expressed in the motor (forelimb and hindlimb areas) and auditory (temporal) cortices, and PCDH9 mRNA was highly expressed in the motor and main somatosensory cortices. These PCDHs were also expressed in the specific regions of the connecting thalamic nuclei. These cortical regionalization and thalamic nuclei-specificity appeared to be most distinct in P3 compared with those of embryonic and adult stages. Taken together, these results suggest that PCDHs may play specific roles in the establishment of selective synaptic connections of specific modality of cerebral cortex with other communicating brain regions such as the thalamus.

Original languageEnglish
Pages (from-to)996-1021
Number of pages26
JournalNeuroscience
Volume147
Issue number4
DOIs
Publication statusPublished - 2007 Jul 29

Fingerprint

Thalamic Nuclei
Messenger RNA
Brain
Cerebral Cortex
Occipital Lobe
Auditory Cortex
Somatosensory Cortex
Forelimb
Visual Cortex
Temporal Lobe
Cadherins
Hindlimb
Thalamus
Cell Adhesion
Nervous System
In Situ Hybridization
Chromosomes
Maintenance
Critical Period (Psychology)

Keywords

  • cell adhesion molecules
  • cerebral cortex
  • development
  • in situ hybridization
  • thalamus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spatiotemporal expression pattern of non-clustered protocadherin family members in the developing rat brain. / Kim, S. Y.; Chung, H. Sun; Sun, Woong; Kim, Hyun.

In: Neuroscience, Vol. 147, No. 4, 29.07.2007, p. 996-1021.

Research output: Contribution to journalArticle

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