TY - GEN
T1 - Spatiotemporally controlled dissolved oxygen concentration gradient generation inside of microfluidic chip without gas supplies
AU - Jang, Heeyeong
AU - Lee, Sang Hoon
N1 - Funding Information:
This work was supported by National Research Foundation of Korea (NRF-2015R1A2A1A09004998), Republic of Korea.
PY - 2016
Y1 - 2016
N2 - DO concentration is known as one of the important microenvironmental factors during stem cell culture because it can decide the destiny of cell like proliferation or remaining stemness. There are some studies to evaluate the impact of DO concentration during stem cell culture by generating DO concentration gradient inside of microfluidic chip. However, these platforms used additional channel structure with gas or scavenging/producing chemicals to diffuse oxygen through cell culture region. Here, we suggest DO generating method inside of PDMS microfluidic chip without any other components like gas channel or chemicals.
AB - DO concentration is known as one of the important microenvironmental factors during stem cell culture because it can decide the destiny of cell like proliferation or remaining stemness. There are some studies to evaluate the impact of DO concentration during stem cell culture by generating DO concentration gradient inside of microfluidic chip. However, these platforms used additional channel structure with gas or scavenging/producing chemicals to diffuse oxygen through cell culture region. Here, we suggest DO generating method inside of PDMS microfluidic chip without any other components like gas channel or chemicals.
KW - Dissloved oxygen concentration gradient
KW - Gas permeability
KW - Oxygen sensor
UR - http://www.scopus.com/inward/record.url?scp=85014259566&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014259566&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014259566
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 391
EP - 392
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -