Abstract
The genus Cercospora contains numerous important plant pathogenic fungi from a diverse range of hosts. Most species of Cercospora are known only from their morphological characters in vivo. Although the genus contains more than 5 000 names, very few cultures and associated DNA sequence data are available. In this study, 360 Cercospora isolates, obtained from 161 host species, 49 host families and 39 countries, were used to compile a molecular phylogeny. Partial sequences were derived from the internal transcribed spacer regions and intervening 5.8S nrRNA, actin, calmodulin, histone H3 and translation elongation factor 1-alpha genes. The resulting phylogenetic clades were evaluated for application of existing species names and five novel species are introduced. Eleven species are epi-, lecto- or neotypified in this study. Although existing species names were available for several clades, it was not always possible to apply North American or European names to African or Asian strains and vice versa. Some species were found to be limited to a specific host genus, whereas others were isolated from a wide host range. No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study.
| Original language | English |
|---|---|
| Pages (from-to) | 115-170 |
| Number of pages | 56 |
| Journal | Studies in Mycology |
| Volume | 75 |
| DOIs | |
| Publication status | Published - 2013 Jun 1 |
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Keywords
- Cercospora apii complex
- Co-evolution, host jumping
- Host specificity
- Speciation
ASJC Scopus subject areas
- Plant Science
Cite this
Species concepts in cercospora : Spotting the weeds among the roses. / Groenewald, J. Z.; Nakashima, C.; Nishikawa, J.; Shin, Hyeon-Dong; Park, J. H.; Jama, A. N.; Groenewald, M.; Braun, U.; Crous, P. W.
In: Studies in Mycology, Vol. 75, 01.06.2013, p. 115-170.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Species concepts in cercospora
T2 - Spotting the weeds among the roses
AU - Groenewald, J. Z.
AU - Nakashima, C.
AU - Nishikawa, J.
AU - Shin, Hyeon-Dong
AU - Park, J. H.
AU - Jama, A. N.
AU - Groenewald, M.
AU - Braun, U.
AU - Crous, P. W.
PY - 2013/6/1
Y1 - 2013/6/1
N2 - The genus Cercospora contains numerous important plant pathogenic fungi from a diverse range of hosts. Most species of Cercospora are known only from their morphological characters in vivo. Although the genus contains more than 5 000 names, very few cultures and associated DNA sequence data are available. In this study, 360 Cercospora isolates, obtained from 161 host species, 49 host families and 39 countries, were used to compile a molecular phylogeny. Partial sequences were derived from the internal transcribed spacer regions and intervening 5.8S nrRNA, actin, calmodulin, histone H3 and translation elongation factor 1-alpha genes. The resulting phylogenetic clades were evaluated for application of existing species names and five novel species are introduced. Eleven species are epi-, lecto- or neotypified in this study. Although existing species names were available for several clades, it was not always possible to apply North American or European names to African or Asian strains and vice versa. Some species were found to be limited to a specific host genus, whereas others were isolated from a wide host range. No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study.
AB - The genus Cercospora contains numerous important plant pathogenic fungi from a diverse range of hosts. Most species of Cercospora are known only from their morphological characters in vivo. Although the genus contains more than 5 000 names, very few cultures and associated DNA sequence data are available. In this study, 360 Cercospora isolates, obtained from 161 host species, 49 host families and 39 countries, were used to compile a molecular phylogeny. Partial sequences were derived from the internal transcribed spacer regions and intervening 5.8S nrRNA, actin, calmodulin, histone H3 and translation elongation factor 1-alpha genes. The resulting phylogenetic clades were evaluated for application of existing species names and five novel species are introduced. Eleven species are epi-, lecto- or neotypified in this study. Although existing species names were available for several clades, it was not always possible to apply North American or European names to African or Asian strains and vice versa. Some species were found to be limited to a specific host genus, whereas others were isolated from a wide host range. No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study.
KW - Cercospora apii complex
KW - Co-evolution, host jumping
KW - Host specificity
KW - Speciation
UR - http://www.scopus.com/inward/record.url?scp=84876320366&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876320366&partnerID=8YFLogxK
U2 - 10.3114/sim0012
DO - 10.3114/sim0012
M3 - Article
VL - 75
SP - 115
EP - 170
JO - Studies in Mycology
JF - Studies in Mycology
SN - 0166-0616
ER -