Species concepts in cercospora

Spotting the weeds among the roses

J. Z. Groenewald, C. Nakashima, J. Nishikawa, Hyeon-Dong Shin, J. H. Park, A. N. Jama, M. Groenewald, U. Braun, P. W. Crous

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

The genus Cercospora contains numerous important plant pathogenic fungi from a diverse range of hosts. Most species of Cercospora are known only from their morphological characters in vivo. Although the genus contains more than 5 000 names, very few cultures and associated DNA sequence data are available. In this study, 360 Cercospora isolates, obtained from 161 host species, 49 host families and 39 countries, were used to compile a molecular phylogeny. Partial sequences were derived from the internal transcribed spacer regions and intervening 5.8S nrRNA, actin, calmodulin, histone H3 and translation elongation factor 1-alpha genes. The resulting phylogenetic clades were evaluated for application of existing species names and five novel species are introduced. Eleven species are epi-, lecto- or neotypified in this study. Although existing species names were available for several clades, it was not always possible to apply North American or European names to African or Asian strains and vice versa. Some species were found to be limited to a specific host genus, whereas others were isolated from a wide host range. No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study.

Original languageEnglish
Pages (from-to)115-170
Number of pages56
JournalStudies in Mycology
Volume75
DOIs
Publication statusPublished - 2013 Jun 1

Fingerprint

Cercospora
Rosa
weeds
host range
loci
genes
phylogeny
DNA barcoding
plant pathogenic fungi
calmodulin
histones
translation (genetics)
internal transcribed spacers
actin
nucleotide sequences

Keywords

  • Cercospora apii complex
  • Co-evolution, host jumping
  • Host specificity
  • Speciation

ASJC Scopus subject areas

  • Plant Science

Cite this

Groenewald, J. Z., Nakashima, C., Nishikawa, J., Shin, H-D., Park, J. H., Jama, A. N., ... Crous, P. W. (2013). Species concepts in cercospora: Spotting the weeds among the roses. Studies in Mycology, 75, 115-170. https://doi.org/10.3114/sim0012

Species concepts in cercospora : Spotting the weeds among the roses. / Groenewald, J. Z.; Nakashima, C.; Nishikawa, J.; Shin, Hyeon-Dong; Park, J. H.; Jama, A. N.; Groenewald, M.; Braun, U.; Crous, P. W.

In: Studies in Mycology, Vol. 75, 01.06.2013, p. 115-170.

Research output: Contribution to journalArticle

Groenewald, JZ, Nakashima, C, Nishikawa, J, Shin, H-D, Park, JH, Jama, AN, Groenewald, M, Braun, U & Crous, PW 2013, 'Species concepts in cercospora: Spotting the weeds among the roses', Studies in Mycology, vol. 75, pp. 115-170. https://doi.org/10.3114/sim0012
Groenewald JZ, Nakashima C, Nishikawa J, Shin H-D, Park JH, Jama AN et al. Species concepts in cercospora: Spotting the weeds among the roses. Studies in Mycology. 2013 Jun 1;75:115-170. https://doi.org/10.3114/sim0012
Groenewald, J. Z. ; Nakashima, C. ; Nishikawa, J. ; Shin, Hyeon-Dong ; Park, J. H. ; Jama, A. N. ; Groenewald, M. ; Braun, U. ; Crous, P. W. / Species concepts in cercospora : Spotting the weeds among the roses. In: Studies in Mycology. 2013 ; Vol. 75. pp. 115-170.
@article{0c527de518be469f8a1b9b2669a493fe,
title = "Species concepts in cercospora: Spotting the weeds among the roses",
abstract = "The genus Cercospora contains numerous important plant pathogenic fungi from a diverse range of hosts. Most species of Cercospora are known only from their morphological characters in vivo. Although the genus contains more than 5 000 names, very few cultures and associated DNA sequence data are available. In this study, 360 Cercospora isolates, obtained from 161 host species, 49 host families and 39 countries, were used to compile a molecular phylogeny. Partial sequences were derived from the internal transcribed spacer regions and intervening 5.8S nrRNA, actin, calmodulin, histone H3 and translation elongation factor 1-alpha genes. The resulting phylogenetic clades were evaluated for application of existing species names and five novel species are introduced. Eleven species are epi-, lecto- or neotypified in this study. Although existing species names were available for several clades, it was not always possible to apply North American or European names to African or Asian strains and vice versa. Some species were found to be limited to a specific host genus, whereas others were isolated from a wide host range. No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study.",
keywords = "Cercospora apii complex, Co-evolution, host jumping, Host specificity, Speciation",
author = "Groenewald, {J. Z.} and C. Nakashima and J. Nishikawa and Hyeon-Dong Shin and Park, {J. H.} and Jama, {A. N.} and M. Groenewald and U. Braun and Crous, {P. W.}",
year = "2013",
month = "6",
day = "1",
doi = "10.3114/sim0012",
language = "English",
volume = "75",
pages = "115--170",
journal = "Studies in Mycology",
issn = "0166-0616",
publisher = "Centraalbureau voor Schimmelculturen",

}

TY - JOUR

T1 - Species concepts in cercospora

T2 - Spotting the weeds among the roses

AU - Groenewald, J. Z.

AU - Nakashima, C.

AU - Nishikawa, J.

AU - Shin, Hyeon-Dong

AU - Park, J. H.

AU - Jama, A. N.

AU - Groenewald, M.

AU - Braun, U.

AU - Crous, P. W.

PY - 2013/6/1

Y1 - 2013/6/1

N2 - The genus Cercospora contains numerous important plant pathogenic fungi from a diverse range of hosts. Most species of Cercospora are known only from their morphological characters in vivo. Although the genus contains more than 5 000 names, very few cultures and associated DNA sequence data are available. In this study, 360 Cercospora isolates, obtained from 161 host species, 49 host families and 39 countries, were used to compile a molecular phylogeny. Partial sequences were derived from the internal transcribed spacer regions and intervening 5.8S nrRNA, actin, calmodulin, histone H3 and translation elongation factor 1-alpha genes. The resulting phylogenetic clades were evaluated for application of existing species names and five novel species are introduced. Eleven species are epi-, lecto- or neotypified in this study. Although existing species names were available for several clades, it was not always possible to apply North American or European names to African or Asian strains and vice versa. Some species were found to be limited to a specific host genus, whereas others were isolated from a wide host range. No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study.

AB - The genus Cercospora contains numerous important plant pathogenic fungi from a diverse range of hosts. Most species of Cercospora are known only from their morphological characters in vivo. Although the genus contains more than 5 000 names, very few cultures and associated DNA sequence data are available. In this study, 360 Cercospora isolates, obtained from 161 host species, 49 host families and 39 countries, were used to compile a molecular phylogeny. Partial sequences were derived from the internal transcribed spacer regions and intervening 5.8S nrRNA, actin, calmodulin, histone H3 and translation elongation factor 1-alpha genes. The resulting phylogenetic clades were evaluated for application of existing species names and five novel species are introduced. Eleven species are epi-, lecto- or neotypified in this study. Although existing species names were available for several clades, it was not always possible to apply North American or European names to African or Asian strains and vice versa. Some species were found to be limited to a specific host genus, whereas others were isolated from a wide host range. No single locus was found to be the ideal DNA barcode gene for the genus, and species identification needs to be based on a combination of gene loci and morphological characters. Additional primers were developed to supplement those previously published for amplification of the loci used in this study.

KW - Cercospora apii complex

KW - Co-evolution, host jumping

KW - Host specificity

KW - Speciation

UR - http://www.scopus.com/inward/record.url?scp=84876320366&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876320366&partnerID=8YFLogxK

U2 - 10.3114/sim0012

DO - 10.3114/sim0012

M3 - Article

VL - 75

SP - 115

EP - 170

JO - Studies in Mycology

JF - Studies in Mycology

SN - 0166-0616

ER -