Specific antioxidant selenoproteins are induced in the heart during hypertrophy

Fukun W. Hoffmann, Ann S. Hashimoto, Byung Cheon Lee, Aaron H. Rose, Ralph V. Shohet, Peter R. Hoffmann

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Selenium (Se) is thought to confer cardioprotective effects through the actions of antioxidant selenoprotein enzymes that directly limit levels of ROS such as hydrogen peroxide (H2O2) or that reverse oxidative damage to lipids and proteins. To determine how the selenoproteome responds to myocardial hypertrophy, two mouse models were employed: triidothyronine (T3)- or isoproterenol (ISO)-treatment. After 7 days of T3- and ISO-treatment, cardiac stress was demonstrated by increased H2O2 and caspase-3 activity. Neither treatment produced significant increases in phospholipid peroxidation or TUNEL-positive cells, suggesting that antioxidant systems were protecting the cardiomyocytes from damage. Many selenoprotein mRNAs were induced by T3- and ISO-treatment, with levels of methionine sulfoxide reductase 1 (MsrB1, also called SelR) mRNA showing the largest increases. MsrB enzymatic activity was also elevated in both models of cardiac stress, while glutathione peroxidase (GPx) activity and thioredoxin reductase (Trxrd) activity were moderately and nonsignificantly increased, respectively. Western blot assays revealed a marked increase in MsrB1 and moderate increases in GPx3, GPx4, and Trxrd1, particularly in T3-treated hearts. Thus, the main response of the selenoproteome during hypertrophy does not involve increased GPx1, but increased GPx3 for reducing extracellular H2O2 and increased GPx4, Trxrd1, and MsrB1 for minimizing intracellular oxidative damage.

Original languageEnglish
Pages (from-to)38-44
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume512
Issue number1
DOIs
Publication statusPublished - 2011 Aug 1

Keywords

  • Antioxidant
  • Cardiac stress
  • Hypertrophy
  • Oxidative stress
  • Selenoproteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Specific antioxidant selenoproteins are induced in the heart during hypertrophy'. Together they form a unique fingerprint.

  • Cite this