Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip

Yeon Seok Kim, Javed H. Niazi, Man Bock Gu

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

An electrochemical sensing system for oxytetracycline (OTC) detection was developed using ssDNA aptamer immobilized on gold interdigitated array (IDA) electrode chip. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). The immobilized thiol-modified aptamer on gold electrode chip served as a biorecognition element for the target molecules and the electrochemical signals generated from interactions between the aptamers and the target molecules was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The current decrease due to the interference of bound OTC, DOX or TET was analyzed with the electron flow produced by a redox reaction between ferro- and ferricyanide. The specificity of developed EC-biosensor for OTC was highly distinguishable from the structurally similar antibiotics (DOX and TET). The dynamic range was determined to be 1-100 nM of OTC concentration in semi-logarithmic coordinates.

Original languageEnglish
Pages (from-to)250-254
Number of pages5
JournalAnalytica Chimica Acta
Volume634
Issue number2
DOIs
Publication statusPublished - 2009 Feb 23

Keywords

  • Aptamer
  • Electrochemical detection
  • Interdigitated array electrode
  • Oxytetracycline

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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