Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells

Seung Cheol Choi; Wan Joo Shim; Do Sun Lim

doi:10.1007/s10529-007-9631-z

Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells

Seung Cheol Choi, Wan Joo Shim, Do Sun Lim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Vectors encoding reporter genes driven by cardiac specific myosin light chain-2v (MLC-2v), endothelial cell-specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation-monitoring vector (pMLC-2v-DsRed), endothelial cell-specific monitoring vectors (pFlk1-EGFP, pTie2-EGFP) as well as the dual promoter driven-reporter genes (pMLC-2v-DsRed-Flk1-EGFP, pMLC-2v-DsRed-Tie2-EGFP) were specifically expressed in the Sca-1⁺ bone marrow mesenchymal stem cells (BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell-specific promoter-driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.

Original language	English
Pages (from-to)	835-843
Number of pages	9
Journal	Biotechnology letters
Volume	30
Issue number	5
DOIs	https://doi.org/10.1007/s10529-007-9631-z
Publication status	Published - 2008 May

Keywords

Cardiomyogenic differentiation
Dual promoters
Endothelial differentiation
Mesenchymal stem cells
Reporter genes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-007-9631-z

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@article{36d9148f8c014523a99911f91f02a421,

title = "Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells",

abstract = "Vectors encoding reporter genes driven by cardiac specific myosin light chain-2v (MLC-2v), endothelial cell-specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation-monitoring vector (pMLC-2v-DsRed), endothelial cell-specific monitoring vectors (pFlk1-EGFP, pTie2-EGFP) as well as the dual promoter driven-reporter genes (pMLC-2v-DsRed-Flk1-EGFP, pMLC-2v-DsRed-Tie2-EGFP) were specifically expressed in the Sca-1+ bone marrow mesenchymal stem cells (BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell-specific promoter-driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.",

keywords = "Cardiomyogenic differentiation, Dual promoters, Endothelial differentiation, Mesenchymal stem cells, Reporter genes",

author = "Choi, {Seung Cheol} and Shim, {Wan Joo} and Lim, {Do Sun}",

note = "Funding Information: Acknowledgements This research was supported by a grant (SC3170) from the Stem Cell Research Center of the 21st Century Frontier Research Program, which is funded by the Ministry of Science and Technology.",

year = "2008",

month = may,

doi = "10.1007/s10529-007-9631-z",

language = "English",

volume = "30",

pages = "835--843",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Netherlands",

number = "5",

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TY - JOUR

T1 - Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells

AU - Choi, Seung Cheol

AU - Shim, Wan Joo

AU - Lim, Do Sun

N1 - Funding Information: Acknowledgements This research was supported by a grant (SC3170) from the Stem Cell Research Center of the 21st Century Frontier Research Program, which is funded by the Ministry of Science and Technology.

PY - 2008/5

Y1 - 2008/5

N2 - Vectors encoding reporter genes driven by cardiac specific myosin light chain-2v (MLC-2v), endothelial cell-specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation-monitoring vector (pMLC-2v-DsRed), endothelial cell-specific monitoring vectors (pFlk1-EGFP, pTie2-EGFP) as well as the dual promoter driven-reporter genes (pMLC-2v-DsRed-Flk1-EGFP, pMLC-2v-DsRed-Tie2-EGFP) were specifically expressed in the Sca-1+ bone marrow mesenchymal stem cells (BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell-specific promoter-driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.

AB - Vectors encoding reporter genes driven by cardiac specific myosin light chain-2v (MLC-2v), endothelial cell-specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation-monitoring vector (pMLC-2v-DsRed), endothelial cell-specific monitoring vectors (pFlk1-EGFP, pTie2-EGFP) as well as the dual promoter driven-reporter genes (pMLC-2v-DsRed-Flk1-EGFP, pMLC-2v-DsRed-Tie2-EGFP) were specifically expressed in the Sca-1+ bone marrow mesenchymal stem cells (BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell-specific promoter-driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.

KW - Cardiomyogenic differentiation

KW - Dual promoters

KW - Endothelial differentiation

KW - Mesenchymal stem cells

KW - Reporter genes

UR - http://www.scopus.com/inward/record.url?scp=41549150364&partnerID=8YFLogxK

U2 - 10.1007/s10529-007-9631-z

DO - 10.1007/s10529-007-9631-z

M3 - Article

C2 - 18175067

AN - SCOPUS:41549150364

SN - 0141-5492

VL - 30

SP - 835

EP - 843

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 5

ER -

Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells

Abstract

Keywords

ASJC Scopus subject areas

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