Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells

Seung Cheol Choi, Wan Joo Shim, Do-Sun Lim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Vectors encoding reporter genes driven by cardiac specific myosin light chain-2v (MLC-2v), endothelial cell-specific Flk1 or Tie2 promoters were constructed. The cardiac differentiation-monitoring vector (pMLC-2v-DsRed), endothelial cell-specific monitoring vectors (pFlk1-EGFP, pTie2-EGFP) as well as the dual promoter driven-reporter genes (pMLC-2v-DsRed-Flk1-EGFP, pMLC-2v-DsRed-Tie2-EGFP) were specifically expressed in the Sca-1 + bone marrow mesenchymal stem cells (BMMSCs) with cardiomyogenic or endothelial lineage differentiation. The cardiac or endothelial cell-specific promoter-driven reporter vectors provide important tools for the study of stem cell fate and differentiation in vitro and future stem cell therapy for ischemic cardiomyopathy.

Original languageEnglish
Pages (from-to)835-843
Number of pages9
JournalBiotechnology Letters
Volume30
Issue number5
DOIs
Publication statusPublished - 2008 May 1

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Endothelial cells
Bone
Endothelial Cells
Bone Marrow
Reporter Genes
Monitoring
Stem Cells
Cardiac Myosins
Myosin Light Chains
Cell- and Tissue-Based Therapy
Cardiomyopathies
Gene encoding
Cell Differentiation
Genes
fluorescent protein 583

Keywords

  • Cardiomyogenic differentiation
  • Dual promoters
  • Endothelial differentiation
  • Mesenchymal stem cells
  • Reporter genes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells. / Choi, Seung Cheol; Shim, Wan Joo; Lim, Do-Sun.

In: Biotechnology Letters, Vol. 30, No. 5, 01.05.2008, p. 835-843.

Research output: Contribution to journalArticle

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