Specific responses of bacterial cells to dioxins

Jiho Min, Chi Hoa Pham, Man Bock Gu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Five different recombinant bioluminescent strains of Escherichia coli that contain the recA (responsive to DNA damage related stress), fabA (membrane damage), katG (oxidative damage), grpE (protein damage), and lac (constitutive expression, general toxicity) promoters fused to the bacterial lux operon from either Vibrio fischeri or Photorhabdus luminescens were used to describe the different mechanisms of toxicity that several dibenzo-p-dioxins and dibenzofurans have on bacteria, as well as to determine whether bacteria can sensitively detect the presence of these compounds. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) was found to cause only DNA-related damage to bacterial cells. However, the four stress-responsive strains showed positive responses after addition of 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-TCDD), whereas 2,3,7,8-tetrachlorodibenzo-p-furan (2,3,7,8-TCDF) caused only DNA, oxidative, and protein damage. However, 2,8-dichlorodibenzo-p-dioxin (2,8-DCDD) was not found to induce any stresses tested for in this study, that is, DNA, membrane, oxidative, and protein damage, indicating that each congener might differentially interact with the cell, stimulating differential stress responses within them. By using the constitutive strain, we found that the level of cellular toxicity experienced due to the addition of these four dioxins decreased in the order of 2,3,7,8-TCDD (the most toxic), 1,2,3,4-TCDD, 2,8-DCDD, and 2,3,7,8-TCDF. The 20% effective concentration (EC20), defined in this study the concentration of chemical that causes a 20% decrease in the bioluminescence 60 min after induction, was only 0.1 μg/L for 2,3,7,8-TCDD, a value that is lower than that of the other congeners and demonstrates that 2,3,7,8-TCDD was the most toxic compound tested in this study.

Original languageEnglish
Pages (from-to)233-238
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume22
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1
Externally publishedYes

Fingerprint

Dioxins
TCDD
dioxin
Toxicity
damage
Poisons
DNA
Bacteria
DNA Damage
Bioluminescence
Membranes
Photorhabdus
Proteins
Aliivibrio fischeri
toxicity
protein
Escherichia coli
Operon
membrane
bioluminescence

Keywords

  • Dioxins
  • Recombinant bioluminescent bacteria
  • Stress response
  • Toxicity

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Specific responses of bacterial cells to dioxins. / Min, Jiho; Pham, Chi Hoa; Gu, Man Bock.

In: Environmental Toxicology and Chemistry, Vol. 22, No. 2, 01.02.2003, p. 233-238.

Research output: Contribution to journalArticle

Min, Jiho ; Pham, Chi Hoa ; Gu, Man Bock. / Specific responses of bacterial cells to dioxins. In: Environmental Toxicology and Chemistry. 2003 ; Vol. 22, No. 2. pp. 233-238.
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