Speckle-field digital holographic microscopy

Yong Keun Park, Wonshik Choi, Zahid Yaqoob, Ramachandra Dasari, Kamran Badizadegan, Michael S. Feld

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

Original languageEnglish
Pages (from-to)12285-12292
Number of pages8
JournalOptics Express
Volume17
Issue number15
DOIs
Publication statusPublished - 2009 Jul 20

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microscopy
coherent light
cells
spatial resolution
recording
illumination
lenses
scanning
electric fields
sensitivity
diffraction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Park, Y. K., Choi, W., Yaqoob, Z., Dasari, R., Badizadegan, K., & Feld, M. S. (2009). Speckle-field digital holographic microscopy. Optics Express, 17(15), 12285-12292. https://doi.org/10.1364/OE.17.012285

Speckle-field digital holographic microscopy. / Park, Yong Keun; Choi, Wonshik; Yaqoob, Zahid; Dasari, Ramachandra; Badizadegan, Kamran; Feld, Michael S.

In: Optics Express, Vol. 17, No. 15, 20.07.2009, p. 12285-12292.

Research output: Contribution to journalArticle

Park, YK, Choi, W, Yaqoob, Z, Dasari, R, Badizadegan, K & Feld, MS 2009, 'Speckle-field digital holographic microscopy', Optics Express, vol. 17, no. 15, pp. 12285-12292. https://doi.org/10.1364/OE.17.012285
Park YK, Choi W, Yaqoob Z, Dasari R, Badizadegan K, Feld MS. Speckle-field digital holographic microscopy. Optics Express. 2009 Jul 20;17(15):12285-12292. https://doi.org/10.1364/OE.17.012285
Park, Yong Keun ; Choi, Wonshik ; Yaqoob, Zahid ; Dasari, Ramachandra ; Badizadegan, Kamran ; Feld, Michael S. / Speckle-field digital holographic microscopy. In: Optics Express. 2009 ; Vol. 17, No. 15. pp. 12285-12292.
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