Speckle-field digital holographic microscopy

Yong Keun Park; Wonshik Choi; Zahid Yaqoob; Ramachandra Dasari; Kamran Badizadegan; Michael S. Feld

doi:10.1117/12.842799

Speckle-field digital holographic microscopy

Yong Keun Park, Wonshik Choi, Zahid Yaqoob, Ramachandra Dasari, Kamran Badizadegan, Michael S. Feld

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging (For the detail, please see the Ref. [1]). A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs	https://doi.org/10.1117/12.842799
Publication status	Published - 2010
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States Duration: 2010 Jan 23 → 2010 Jan 25

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7568
ISSN (Print)	1605-7422

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Country/Territory	United States
City	San Francisco, CA
Period	10/1/23 → 10/1/25

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.842799

Cite this

Park, YK, Choi, W, Yaqoob, Z, Dasari, R, Badizadegan, K & Feld, MS 2010, Speckle-field digital holographic microscopy. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII., 75681O, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.842799

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AU - Badizadegan, Kamran

AU - Feld, Michael S.

PY - 2010

Y1 - 2010

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AB - We report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging (For the detail, please see the Ref. [1]). A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

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M3 - Conference contribution

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

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ER -

Speckle-field digital holographic microscopy

Abstract

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