Speckle-field digital holographic microscopy

Yong Keun Park, Wonshik Choi, Zahid Yaqoob, Ramachandra Dasari, Kamran Badizadegan, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging (For the detail, please see the Ref. [1]). A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs
Publication statusPublished - 2010
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7568
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Speckle-field digital holographic microscopy'. Together they form a unique fingerprint.

  • Cite this

    Park, Y. K., Choi, W., Yaqoob, Z., Dasari, R., Badizadegan, K., & Feld, M. S. (2010). Speckle-field digital holographic microscopy. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII [75681O] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568). https://doi.org/10.1117/12.842799