Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

YongKeun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, Michael S. Feld

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.

Original languageEnglish
Pages (from-to)3668-3670
Number of pages3
JournalOptics Letters
Volume34
Issue number23
DOIs
Publication statusPublished - 2009 Dec 1

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hemoglobin
erythrocytes
Microscopy
Hemoglobins
Erythrocytes
microscopy
Physiologic Monitoring
albumins
wavelengths
Calibration
Albumins
light sources
phase shift
Light
cells
diffraction
molecules

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. / Park, YongKeun; Yamauchi, Toyohiko; Choi, Wonshik; Dasari, Ramachandra; Feld, Michael S.

In: Optics Letters, Vol. 34, No. 23, 01.12.2009, p. 3668-3670.

Research output: Contribution to journalArticle

Park, YongKeun ; Yamauchi, Toyohiko ; Choi, Wonshik ; Dasari, Ramachandra ; Feld, Michael S. / Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. In: Optics Letters. 2009 ; Vol. 34, No. 23. pp. 3668-3670.
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