Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

Yong Keun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sampleinduced phase shifts (for the detail, please see the Ref. [1]). Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual red blood cells (RBCs). The end point of this study is non-invasive monitoring of physiological states of intact living cells.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs
Publication statusPublished - 2010
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7568
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Park, Y. K., Yamauchi, T., Choi, W., Dasari, R., & Feld, M. S. (2010). Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII [756812] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568). https://doi.org/10.1117/12.842813