Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

YongKeun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sampleinduced phase shifts (for the detail, please see the Ref. [1]). Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual red blood cells (RBCs). The end point of this study is non-invasive monitoring of physiological states of intact living cells.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7568
DOIs
Publication statusPublished - 2010 May 10
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States
Duration: 2010 Jan 232010 Jan 25

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
CountryUnited States
CitySan Francisco, CA
Period10/1/2310/1/25

Fingerprint

Hemoglobin
hemoglobin
erythrocytes
Microscopy
Microscopic examination
Hemoglobins
Blood
Erythrocytes
Cells
microscopy
Wavelength
Physiologic Monitoring
albumins
Phase shift
wavelengths
Calibration
Light sources
Labels
Albumins
light sources

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Park, Y., Yamauchi, T., Choi, W., Dasari, R., & Feld, M. S. (2010). Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7568). [756812] https://doi.org/10.1117/12.842813

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. / Park, YongKeun; Yamauchi, Toyohiko; Choi, Wonshik; Dasari, Ramachandra; Feld, Michael S.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7568 2010. 756812.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Park, Y, Yamauchi, T, Choi, W, Dasari, R & Feld, MS 2010, Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7568, 756812, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.842813
Park Y, Yamauchi T, Choi W, Dasari R, Feld MS. Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7568. 2010. 756812 https://doi.org/10.1117/12.842813
Park, YongKeun ; Yamauchi, Toyohiko ; Choi, Wonshik ; Dasari, Ramachandra ; Feld, Michael S. / Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7568 2010.
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