Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice

Hyeon Son Choi, Eu Ddeum Park, Yooheon Park, Hyung Joo Suh

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The aim of this study is to evaluate the effect of spent coffee ground (SCG) ethanol extract on UVB-induced skin aging in hairless mice. An ethanol extract of SCG (ESCG) was prepared using the residue remaining after extraction of oil from roasted SCG. High performance liquid chromatography (HPLC) analysis showed that the content of caffeine (41.58 ± 0.54 μg/mg) was higher than that of chlorogenic acid isomers (~ 9.17 μg/mg) in ESCG. ESCG significantly decreased the UVB-induced intracellular reactive oxygen species in HaCaT cells. UVB-induced wrinkle formation in mice dorsal skin was effectively reduced by ESCG administration; high dose of ESCG (5 g/L) caused the reduction of wrinkle area by 30% compared with UVB-treated control (UVBC). This result correlated with the ESCG-mediated decrease in epidermis thickness (25%). In addition, ESCG administration significantly reduced transdermal water loss (20%) and erythema formation (35%) derived from UVB exposure. Collagen type I (COL-1) level in dorsal skin was effectively recovered by ESCG administration. These results were supported by down-regulation of collagen-degrading matrix metalloproteinase 2 (MMP2) and 9 (MMP9) expressions. Our results indicate that ESCG protects mouse skin from UVB-induced photoaging by suppressing the expression of matrix metalloproteinases. Our study suggests that ESCG may be anti-photoaging agent.

Original languageEnglish
Pages (from-to)164-172
Number of pages9
JournalJournal of Photochemistry and Photobiology B: Biology
Volume153
DOIs
Publication statusPublished - 2015 Dec 1

Fingerprint

coffee
Hairless Mouse
Coffee
mice
collagens
Skin
ethyl alcohol
Ethanol
water loss
Skin Aging
caffeine
Chlorogenic Acid
epidermis
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
liquid chromatography
Erythema
matrices
Collagen Type I
Caffeine

Keywords

  • HaCaT cell
  • Hairless mouse
  • ROS scavenging activity
  • Spent coffee ground
  • UVB-induced photoaging

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice. / Choi, Hyeon Son; Park, Eu Ddeum; Park, Yooheon; Suh, Hyung Joo.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 153, 01.12.2015, p. 164-172.

Research output: Contribution to journalArticle

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