Spheroid formation of hepatocarcinoma cells in microwells: Experiments and Monte Carlo simulations

Yan Wang, Myung Hee Kim, Seyed R. Tabaei, Jae Hyeok Park, Kyuhwan Na, Seok Chung, Vladimir P. Zhdanov, Nam Joon Cho

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated lowadhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.

Original languageEnglish
Article numbere0161915
JournalPLoS One
Volume11
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1

Fingerprint

Cell proliferation
Cells
Silicone Elastomers
Scaffolds
Actins
cell aggregates
Gravitation
Adhesion
Experiments
cells
cell proliferation
Cell Proliferation
human cell lines
silicone
Population Growth
Cell Adhesion
gravity
Cell Division
fluorescent antibody technique
actin

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Spheroid formation of hepatocarcinoma cells in microwells : Experiments and Monte Carlo simulations. / Wang, Yan; Kim, Myung Hee; Tabaei, Seyed R.; Park, Jae Hyeok; Na, Kyuhwan; Chung, Seok; Zhdanov, Vladimir P.; Cho, Nam Joon.

In: PLoS One, Vol. 11, No. 8, e0161915, 01.08.2016.

Research output: Contribution to journalArticle

Wang, Y, Kim, MH, Tabaei, SR, Park, JH, Na, K, Chung, S, Zhdanov, VP & Cho, NJ 2016, 'Spheroid formation of hepatocarcinoma cells in microwells: Experiments and Monte Carlo simulations', PLoS One, vol. 11, no. 8, e0161915. https://doi.org/10.1371/journal.pone.0161915
Wang, Yan ; Kim, Myung Hee ; Tabaei, Seyed R. ; Park, Jae Hyeok ; Na, Kyuhwan ; Chung, Seok ; Zhdanov, Vladimir P. ; Cho, Nam Joon. / Spheroid formation of hepatocarcinoma cells in microwells : Experiments and Monte Carlo simulations. In: PLoS One. 2016 ; Vol. 11, No. 8.
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