TY - JOUR
T1 - Sphingomonas changnyeongensis sp. Nov. isolated from the hapcheon–changnyeong barrage area in the nakdong river
AU - Park, Chulwoo
AU - Kim, Minkyung
AU - Lee, Byoung Hee
AU - Lee, Ki Eun
AU - Park, Woojun
N1 - Funding Information:
This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108). This work is also supported by a Korea University Grant (K2006821).
PY - 2020
Y1 - 2020
N2 - The novel bacterial strain C33T was isolated from a freshwater sample collected from the Hapcheon–Changnyeong barrage. The Gram-negative, motile, yellow-pigmented strain C33T was characterized as a rod-shaped and strictly aerobic bacterium. A 16S-rRNA phylogenetic analysis revealed that this strain was most closely related to Sphingomonas changbaiensis V2M44T, Sphingomonas tabacisoli X1-8T, and Sphingomonas flavalba ZLT-5T with 97.1, 97.0, and 95.0% 16S-rRNA sequence similari-ties, respectively. The genomic DNA GC content of strain C33T was estimated at 65.0 mol%. The average nucleotide identity of strain C33T relative to S. changbaiensis V2M44T and S. flavalba ZLT-5T was found to be 77.0 and 75.6%, with average amino-acid identities of 69.9, and 66.7%, and the digital DNA–DNA hybridization values of 21.3 and 17.7%, respectively. The cells grew at 19–37 °C and pH 6–9 with 0–0.5% (w/v) NaCl (optimum: 28 °C, pH 6.5, and 0% NaCl). The major component identified in the polyamine pattern was sym-homospermidine, and the main ubiquinone was Q-10. The predominant polar lipids characterized were diphophatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phos-phatidyldimethylethanolamine, and sphingoglycolipid. Iso-C15:0, C15:0 anteiso, and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c) were found to be the primary cellular fatty acids in strain C33T. Based on these genotypic and phenotypic characteristics, strain C33T was classified as a novel species of the genus Sphingomonas; and the name Sphingomonas changnyeongensis sp. nov. is proposed (=KACC 21511T=JCM 33880T).
AB - The novel bacterial strain C33T was isolated from a freshwater sample collected from the Hapcheon–Changnyeong barrage. The Gram-negative, motile, yellow-pigmented strain C33T was characterized as a rod-shaped and strictly aerobic bacterium. A 16S-rRNA phylogenetic analysis revealed that this strain was most closely related to Sphingomonas changbaiensis V2M44T, Sphingomonas tabacisoli X1-8T, and Sphingomonas flavalba ZLT-5T with 97.1, 97.0, and 95.0% 16S-rRNA sequence similari-ties, respectively. The genomic DNA GC content of strain C33T was estimated at 65.0 mol%. The average nucleotide identity of strain C33T relative to S. changbaiensis V2M44T and S. flavalba ZLT-5T was found to be 77.0 and 75.6%, with average amino-acid identities of 69.9, and 66.7%, and the digital DNA–DNA hybridization values of 21.3 and 17.7%, respectively. The cells grew at 19–37 °C and pH 6–9 with 0–0.5% (w/v) NaCl (optimum: 28 °C, pH 6.5, and 0% NaCl). The major component identified in the polyamine pattern was sym-homospermidine, and the main ubiquinone was Q-10. The predominant polar lipids characterized were diphophatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phos-phatidyldimethylethanolamine, and sphingoglycolipid. Iso-C15:0, C15:0 anteiso, and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c) were found to be the primary cellular fatty acids in strain C33T. Based on these genotypic and phenotypic characteristics, strain C33T was classified as a novel species of the genus Sphingomonas; and the name Sphingomonas changnyeongensis sp. nov. is proposed (=KACC 21511T=JCM 33880T).
KW - Alphaproteobacteria
KW - Freshwater
KW - Sphingoglycolipid
KW - Sphingomonas
KW - Taxonomy
KW - Ubiquinone Q10
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U2 - 10.1099/ijsem.0.004503
DO - 10.1099/ijsem.0.004503
M3 - Article
C2 - 33048038
AN - SCOPUS:85098252262
VL - 70
SP - 6091
EP - 6097
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 12
M1 - 004503
ER -