Sphingosine-1-phosphate-induced intracellular Ca2+ mobilization in human endothelial cells

Geun Hee Seol, Moon Y. Kim, Guo Hua Liang, Ji Aee Kim, Young Ju Kim, Seikwan Oh, Suk Hyo Suh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The authors have studied the effect of sphingosine-1-phosphate (S1P) on Ca2+ release from intracellular stores in cultured human umbilical vein endothelial cells (HUVECs). In the presence of extracellular Ca2+, S1P increased intracellular Ca2+ concentration ([Ca2+]i) and this increase was partially inhibited by La3+ (1 μM), indicating that S1P induces Ca2+ influx from extracellular pool and Ca2+ release from intracellular stores. S1P increased [Ca2+]i concentration dependently in Ca2+-free extracellular solution. The Hill coefficient (1.7) and EC50 (420 nM) was obtained from the concentration-response relationship. When caffeine depleted Ca2+ store in the presence of ryanodine, S1P did not induce intracellular Ca2+ release. Furthermore, the Ca2+-induced Ca2+ release inhibitors ruthenium red or dantrolene completely inhibited S1P-induced intracellular Ca2+ release. S1P-induced intracellular Ca2+ release was inhibited by the phospholipase C (PLC) inhibitors neomycin and U73312, or the inositol 1,4,5-triphosphate (IP3)-gated Ca2+ channel blocker aminoethoxybiphenyl borane (2-APB). In contrast, S1P-induced intracellular Ca2+ release was not inhibited by the mitochondrial Ca2+ uptake inhibitor CCCP or the mitochondrial Ca2+ release inhibitor cyclosporin A. These results show that S1P mobilizes Ca 2+ from intracellular stores primarily via Ca2+-induced and IP3-induced Ca 2+ release and this Ca2+ mobilization is independent of mitochondrial Ca2+ stores.

Original languageEnglish
Pages (from-to)263-269
Number of pages7
JournalEndothelium: Journal of Endothelial Cell Research
Volume12
Issue number5-6
DOIs
Publication statusPublished - 2005 Sep
Externally publishedYes

Keywords

  • Ca-induced Ca release
  • Human umbilical vein endothelial cells
  • IP-induced Ca release
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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